Effects of adenylates on the circadian interaction of KaiB with the KaiC complex in the reconstituted cyanobacterial Kai protein oscillator
Effects of adenylates on the circadian interaction of KaiB with the KaiC complex in the reconstituted cyanobacterial Kai protein oscillator
复制标题
腺苷酸对重构蓝藻 Kai 蛋白振荡器中 KaiB 与 KaiC 复合物昼夜相互作用的影响
DOI：
10.1080/09168451.2014.940833
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Oyama T
中科院分区：
文献类型：
--
作者：
Goda K;Kondo T;Oyama T
蓝藻是具有昼夜节律振荡器的光合原核生物。时钟蛋白KAIa、Kaib、KaiC组成中央昼夜节律振荡器，在有ATP存在的情况下可在体外重组。KaiC具有ATPase、自体激酶和自磷酸酶活性。这些活性受KAI蛋白之间的蛋白质-蛋白质相互作用调节。在重组系统中，Kaib与KaiC复合体的相互作用表现出昼夜节律。我们之前开发了一个使用荧光相关光谱对该络合物的动态行为进行定量、实时监测的系统。在这里，我们研究了ATP和ADP对Kaib节律性相互作用的影响。我们发现，随着ATP或ADP浓度的增加，细胞周期缩短。将ADP加入到KAI蛋白的振荡中，使其以一种相位依赖的方式移动。这些结果提供了对昼夜振荡携带机制如何与细胞新陈代谢相关联的洞察。
Cyanobacteria are photosynthetic prokaryotes that possess circadian oscillators. Clock proteins, KaiA, KaiB, KaiC compose the central circadian oscillator, which can be reconstitutedin vitroin the presence of ATP. KaiC has ATPase, autokinase, and autophosphatase enzymatic activities. These activities are modulated by protein–protein interactions among the Kai proteins. The interaction of KaiB with the KaiC complex shows a circadian rhythm in the reconstituted system. We previously developed a quantitative, real-time monitoring system for the dynamic behavior of the complex using fluorescence correlation spectroscopy. Here, we examined the effects of ATP and ADP on the rhythmic interaction of KaiB. We show that increased concentration of ATP or ADP shortened period length. Adding ADP to the Kai protein oscillation shifted its phase in a phase-dependent manner. These results provide insight into how circadian oscillation entrainment mechanism is linked to cellular metabolism.