Expression of the guanine nucleotide exchange factor, mr-gef, is regulated during the differentiation of specific subsets of telencephalic neurons
Expression of the guanine nucleotide exchange factor, mr-gef, is regulated during the differentiation of specific subsets of telencephalic neurons
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DOI：
10.1016/j.devbrainres.2003.09.017
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发表时间：
2003-12-19
期刊：
影响因子：
--
通讯作者：
Williams, BP
中科院分区：
文献类型：
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作者：
Bithell, A;Alberta, J;Williams, BP
我们已经进行了一个屏幕相结合的消减杂交与PCR分离基因的调节时，神经上皮（NE）细胞分化成神经元。从这个屏幕上，我们已经分离出一些已知的基因，以前没有与神经发生，以及几个新的基因。在这里，我们报告说，这些基因之一，编码鸟嘌呤核苷酸交换因子（GEF），在不同的神经元群体的分化过程中进行调节。我们已经克隆了大鼠和小鼠的GEF基因，并表明它们是人类基因MR-GEF的直系同源物，MR-GEF编码一种特异性激活小GT3，Rap 1的GEE。因此，我们将大鼠基因命名为大鼠mr gef（rmr-gef），将小鼠基因命名为小鼠mr gef（mmr-gef）。在这里，我们将这两个啮齿动物基因统称为mr-gef表达研究表明，mr-gef是由发育中的啮齿动物中枢神经系统的年轻神经元，但不是由脑室区（VZ）的祖细胞表达。mr-gef在早期端脑神经发生过程中的表达模式与GABA和LIM同源框基因Lhx 6（一种由端脑腹侧产生的GABA能中间神经元表达的转录因子，其中一些在发育过程中迁移到皮层）的表达模式惊人地相似。这些观察结果表明，mr-gef编码一种蛋白质，该蛋白质是参与端脑神经发生-1的信号通路的一部分，特别是在GABA能中间神经元的发育中。(C)2003年爱思唯尔B. V.保留所有战斗。
We have performed a screen combining subtractive hybridization with PCR to isolate genes that are regulated when neuroepithelial (NE) cells differentiate into neurons. From this screen, we have isolated a number of known genes that have not previously been associated with neurogenesis, together with several novel genes. Here we report that one of these genes, encoding a guanine nucleotide exchange factor (GEF), is regulated during the differentiation of distinct neuronal populations. We have cloned both rat and mouse GEF genes and shown that they are orthologs of the human gene, MR-GEF, which encodes a GEE that specifically activates the small GTPase, Rap1. We have therefore named the rat gene rat mr gef (rmr-gef) and the mouse gene mouse mr-gef (mmr-gef). Here, we will collectively refer to these two rodent genes as mr-gef Expression studies show that mr-gef is expressed by young neurons of the developing rodent CNS but not by progenitor cells in the ventricular zone (VZ). The expression pattern of mr-gef during early telencephalic neurogenesis is strikingly similar to that of GABA and the LIM homeobox gene Lhx6, a transcription factor expressed by GABAergic interneurons generated in the ventral telencephalon, some of which migrate into the cortex during development. These observations suggest that mr-gef encodes a protein that is part of a signaling pathway involved in telencephalic neurogenesis-1 particularly in the development of GABAergic interneurons. (C) 2003 Elsevier B.V. All fights reserved.