An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance
An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance
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DOI：
10.1016/j.febslet.2013.04.028
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发表时间：
2013-06-19
期刊：
影响因子：
3.5
通讯作者：
Shin, Jeong Sheop
中科院分区：
文献类型：
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作者：
Cui, Mei Hua;Yoo, Kyoung Shin;Shin, Jeong Sheop
我们已经确定了植物R2 R3-MYB转录因子拟南芥MYB 20（AtMYB 20）的功能。过量表达AtMYB 20的转基因植株（AtMYB 20-OX）耐盐性增强，而阻遏系（AtMYB 20-SRDX）比野生型植株更易受NaCl胁迫。NaCl处理后，AtMYB 20-OX抑制了ABI 1、ABI 2和AtPP 2CA的表达，而AtMYB 20-SRDX诱导了ABI 1、ABI 2和AtPP 2CA的表达。电泳迁移率变动分析结果显示，AtMYB 20结合到包含MYB识别序列（TAACTG）和ABI 1和AtPP 2CA的ACGT核心元件的启动子区域。这些结果表明，AtMYB 20下调PP 2Cs的表达，阿坝信号的负调控，并提高耐盐性。(C)2013年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
We have characterized the function of a plant R2R3-MYB transcription factor, Arabidopsis thaliana MYB20 (AtMYB20). Transgenic plants overexpressing AtMYB20 (AtMYB20-OX) enhanced salt stress tolerance while repression lines (AtMYB20-SRDX) were more vulnerable to NaCl than wild-type plants. Following NaCl treatment, the expressions of ABI1, ABI2 and AtPP2CA, which encode type 2C serine/threonine protein phosphatases (PP2Cs) that act as negative regulators in abscisic acid (ABA) signaling, were suppressed in AtMYB20-OX but induced in AtMYB20-SRDX. The electrophoretic mobility shift assay results revealed that AtMYB20 binds to the promoter regions containing the MYB recognition sequence (TAACTG) and an ACGT core element of ABI1 and AtPP2CA. These findings suggest that AtMYB20 down-regulates the expression of PP2Cs, the negative regulator of ABA signaling, and enhances salt tolerance. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.