Flow cytometry counting of CD34+ cells in whole blood
Flow cytometry counting of CD34+ cells in whole blood
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DOI：
10.1038/77571
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发表时间：
2000-07-01
期刊：
影响因子：
82.9
通讯作者：
Petriz, J
中科院分区：
文献类型：
--
作者：
Fornas, O;Garcia, J;Petriz, J
图 1a，SYTO-13 摄取的动力学测定。使用活 HL60 和 KG1a 细胞对 SYTO-13 摄取进行流式细胞术动力学测定，显示荧光平台随着染料浓度的增加而增加。收集自发荧光 1 分钟，暂停添加 SYTO-13 至终浓度 5、10 和 50 nM。连续荧光记录 10 分钟后停止样品采集。与药物敏感的 HL60 细胞相比，多重耐药 KG1a 细胞对 SYTO-13 的摄取减少。使用碘化丙啶染色将死细胞排除在动力学分析之外。 b 和 c，根据 SYTO-13 核酸染色模式的未裂解脐带血细胞的侧向散射和荧光特性。绿色荧光（FL1）阈值用于排除大多数红细胞和碎片。 b，低侧散射细胞亚群显示 SYTO-13 保留减少。 c, a之后b中相同的单元格
Fig. 1 a, Kinetic determination of SYTO-13 uptake. Flow cytometry kinetic assays of SYTO-13 uptake using living HL60 and KG1a cells, showing an increase of fluorescence plateau with the dye concentration. Autofluorescence was collected for 1 min, with a pause for addition of SYTO-13 to final concentrations of 5, 10 and 50 nM. Sample acquisition was stopped after 10 min of continuous fluorescence registering. Multidrug-resistant KG1a cells showed a reduced SYTO-13 uptake compared with drug-sensitive HL60 cells. Dead cells were excluded from kinetic analysis using propidium iodide staining. b and c, Side-scatter and fluorescent properties of unlysed cord blood cells accordingly to SYTO-13 nucleic acid staining pattern. Green fluorescence (FL1) threshold was used to exclude most erythrocytes and debris. b, Sub-population of low-side scatter cells showing a reduced retention of SYTO-13. c, The same cells in b after a