Astrocyte-endothelial cell relationships during human retinal vascular development
Astrocyte-endothelial cell relationships during human retinal vascular development
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DOI：
10.1167/iovs.03-1169
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发表时间：
2004-06-01
影响因子：
4.4
通讯作者：
Lutty, GA
中科院分区：
文献类型：
--
作者：
Chan-Ling, T;McLeod, DS;Lutty, GA
目的。评估发育中的人类视网膜中血管前体细胞（成血管细胞）和星形胶质细胞前体细胞（APC）存在的证据并确定它们的关系。方法。 Pax-2/GFAP/CD-34 三重标记免疫组织化学应用于 12、14、16 和 20 孕周 (WG) 的四个视网膜，以标记 APC、星形胶质细胞和开放血管。 APC 为 Pax-2(+)/GFAP(-)，而星形胶质细胞为 Pax2(+)/GFAP(+)。腺苷二磷酸酶 (ADPase) 酶组织化学可识别内皮细胞和血管前体细胞，应用于 12、16、17 和 19 WG 的人类视网膜。将尼氏染色（一种非特异性细胞体标记）应用于 14.5、18 和 21 WG 视网膜。使用 CD34 和 ADPase 使建立的血管可视化。 结果。尼氏染色梭形细胞和ADPase(+)血管细胞分布的拓扑分析表明，这两个群体在相应年龄具有相似的分布。 ADPase+ 血管前体细胞领先于未闭血管的前缘超过 1 毫米。相比之下，Pax-2(+)/GFAP(-) APC 以很小的幅度领先于 CD34(+) 血管的前缘，并且定向星形胶质细胞 (Pax-2(+)/GFAP(+)) 与形成的血管和神经纤维束相关。两个 ADPase+ 细胞群是明显的，一个是位于表面的纺锤形细胞群，一个是位于较深的球形细胞群。这些种群的外部界限随着成熟而保持不变。结论。 Pax-2/GFAP/CD34免疫组织化学、Nissl染色和ADPase组织化学的组合表明，使用ADPase和Nissl鉴定的血管前体细胞（成血管细胞）代表了与人视网膜中的Pax-2(+)/GFAP(-) APC不同的群体。这些结果得出这样的结论：最初的人类视网膜脉管系统的形成是通过血管前体细胞先前入侵的血管发生而发生的。
PURPOSE. To evaluate evidence for the presence of vascular precursor cells (angioblasts) and astrocyte precursor cells (APCs) in the developing human retina and determine their relationship.METHODS. Pax-2/GFAP/CD-34 triple-label immunohistochemistry was applied to four retinas aged 12, 14, 16, and 20 weeks of gestation (WG) to label APCs, astrocytes, and patent blood vessels. APCs are Pax-2(+)/GFAP(-), whereas astrocytes are Pax2(+)/GFAP(+). Adenosine diphosphatase (ADPase) enzyme histochemistry, which identifies endothelial cells and vascular precursors, was applied to human retinas aged 12, 16, 17, and 19 WG. Nissl stain, a nonspecific cell soma marker, was applied to 14.5-, 18-, and 21-WG retinas. Established blood vessels were visualized with CD34 and ADPase.RESULTS. Topographical analysis of the distribution of Nissl-stained spindle cells and ADPase(+) vascular cells showed that these two populations have similar distributions at corresponding ages. ADPase+ vascular precursor cells preceded the leading edge of patent vessels by more than 1 millimeter. In contrast, Pax-2(+)/GFAP(-) APCs preceded the leading edge of CD34(+) blood vessels by a very small margin, and committed astrocytes (Pax-2(+)/GFAP(+)) were associated with formed vessels and nerve fiber bundles. Two populations of ADPase+ cells were evident, a spindle-shaped population located superficially and a deeper spherical population. The outer limits of these populations remain static with maturation.CONCLUSIONS. A combination of Pax-2/GFAP/CD34 immunohistochemistry, Nissl staining, and ADPase histochemistry showed that the vascular precursor cells (angioblasts), identified using ADPase and Nissl, represent a population distinct from Pax-2(+)/GFAP(-) APCs in the human retina. These results lead to the conclusion that formation of the initial human retinal vasculature takes place through vasculogenesis from the prior invasion of vascular precursor cells.