A REPEAT BIOPSY STUDY IN SPONTANEOUS CRESCENTIC GLOMERULONEPHRITIS MICE
A REPEAT BIOPSY STUDY IN SPONTANEOUS CRESCENTIC GLOMERULONEPHRITIS MICE
复制标题
自发性新月体肾小球肾炎小鼠的重复活检研究
作者：
S. Miyazawa;K. Saiga;K. Nemoto;T. Mae;O. Hotta
背景资料：自发性新月体肾小球肾炎（SCG）/Kj小鼠是一种理想的人类少免疫性新月体肾小球肾炎（GN）动物模型。在本研究中，我们使用肾活检技术，并分析了新月体形成和肾小球中性粒细胞浸润的SCG/Kj小鼠的时序相关性。方法：通过观察SCG/Kj小鼠肾小球肾炎的发生、发展过程，并进行肾活检。采用酶组织化学和免疫组织化学方法检测病理组织学变化和相应疾病阶段肾小球中性粒细胞内流的动力学变化。结果如下：我们根据尿分析将GN的自然病程分为三个时期：蛋白尿阴性/血尿阴性（P− H−）期，随后是蛋白尿阳性/血尿阴性（P+ H−）期，最后是蛋白尿阳性/血尿阳性（P+ H+）期。P+ H−期存在毛细血管内增殖期，P+ H+期出现新月体形成并延长。在P+ H−期，观察到显著的肾小球中性粒细胞浸润，而这些数量随着新月体形成的进展而减少。结论：这些观察结果表明，SCG/Kj小鼠的尿液分析和组织病理学事件之间存在良好的相关性，并且毛细血管内增殖（包含中性粒细胞浸润）可能有助于这些小鼠中随后的新月体形成。
Background: Spontaneous crescentic glomerulonephritis (SCG)/Kj mice are a candidate for suitable animal model of human pauci-immune crescentic glomerulonephritis (GN). In the present study, we used renal biopsy technique and analyzed time sequence correlations among crescent formation and glomerular neutrophil infiltration in SCG/Kj mice. Methods: We observed the progress of GN in SCG/Kj mice according to the urinary abnormalities, and performed the serial renal biopsies. The kinetics of histopathology and glomerular neutrophil influx corresponding disease stage were examined by enzyme-histochemistry and immunohistochemistry. Results: We divided natural course of GN into three periods in view of urinalysis: a proteinuria-negative/hematuria-negative (P− H−) period, followed by a proteinuria-positive/hematuria-negative (P+ H−) period, and finally a proteinuria-positive/hematuria-positive (P+ H+) period. Endocapillary proliferation phase existed in P+ H− period, whereas crescent formation occurred and extended in P+ H+ period. In P+ H− period, prominent glomerular neutrophil infiltration was observed, while these numbers decreased with the progression of crescent formation. Conclusion: These observations suggest that there is a good correlation between urinalysis and histopathological events of SCG/Kj mice, and that endocapillary proliferation, which contains neutrophil infiltration, may contribute to the subsequent crescent formation in these mice.