Direct effects of prolactin on corticosterone release by zona fasciculata‐reticularis cells from male rats
Direct effects of prolactin on corticosterone release by zona fasciculata‐reticularis cells from male rats
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催乳素对雄性大鼠束状带-网状层细胞释放皮质酮的直接影响
DOI：
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发表时间：
1999
影响因子：
4
通讯作者：
Paulus S. Wang
中科院分区：
文献类型：
--
作者：
Ling;M. Lo;S. Kan;William J Huang;Jiann‐Jong Chen;M. Kau;Jui;Ho Lin;S. Tsai;Y. Chiao;J. Yeh;Wan;Paulus S. Wang
催乳素（PRL）在男性中的作用尚未完全确定。本研究旨在探讨催乳素（PRL）在体外对网状束状核细胞（ZFR）分泌皮质酮的作用及其机制。ZFR细胞从处于正常、高催乳素血症或低催乳素血症情况下的雄性大鼠获得。PRL以剂量依赖性模式刺激正常雄性大鼠ZFR细胞中的皮质酮释放。在存在毛喉素或3-异丁基-1-甲基黄嘌呤（IBMX）的情况下，细胞内腺苷3′-5′-环磷酸（cAMP）浓度与PRL浓度呈正相关。PRL增强cAMP模拟试剂的刺激作用，即，毛喉素、8-溴-腺苷3′，5 ′-环一磷酸（8-Br-cAMP）和IBMX对皮质酮释放的影响。腺苷酸环化酶抑制剂（SQ 22536）抑制皮质酮的释放，尽管存在的PRL。硝苯地平（L型钙通道阻滞剂）不抑制皮质酮释放。采用大鼠垂体前叶（AP）肾被膜下移植的方法，建立高催乳素血症模型。与大脑皮质（CX）移植组相比，AP移植物导致ZFR细胞释放皮质酮、3β-羟基类固醇脱氢酶（HSD）活性和cAMP产量增加。急性低催乳素血症状态诱导溴隐亭2天。结果显示，低催乳素血症组皮质酮的产生低于对照组，并且这种情况随着不同的促肾上腺皮质激素浓度的变化而沿着。这些结果表明，PRL通过cAMP级联和3β-HSD活性增加ZFR细胞释放皮质酮。J.细胞。73：563-572，1999.© 1999 Wiley利斯公司
The role of prolactin (PRL) in the male is not fully defined. The aim of this study was to investigate the function and mechanism of PRL on the production of corticosterone by zona fasciculata‐reticularis (ZFR) cells in vitro. The ZFR cells were obtained from male rats under normal, hyperprolactinemic, or hypoprolactinemic situation. PRL stimulated the corticosterone release in a dose‐dependent pattern in the ZFR cells from normal male rats. The cellular adenosine 3′‐5′‐cyclic monophosphate (cAMP) concentration positively correlated with PRL concentration in the presence of forskolin or 3‐isobutyl‐1‐methylxanthine (IBMX). PRL enhanced the stimulatory effects of cAMP mimetic reagents, i.e., forskolin, 8‐bromo‐adenosine 3′,5′‐cyclic monophosphate (8‐Br‐cAMP), and IBMX on the release of corticosterone. The adenylate cyclase inhibitor (SQ22536) inhibited the corticosterone release in spite of presence of PRL. Nifedipine (L‐type calcium channel blocker) did not inhibit corticosterone release. The hyperprolactinemic condition was actualized by transplantation of donor rat anterior pituitary glands (APs) under kidney capsule. By comparison with the cerebral cortex (CX)‐grafted group, AP‐graft resulted in an increased release of corticosterone, 3β‐hydroxysteriod dehydrogenase (HSD) activity and cAMP production by ZFR cells. Acute hypoprolactinemic status was induced by bromocriptine for 2 days. The results showed the productions of corticosterone were lower in hypoprolactinemic group than in control group, which were persistent along with different ACTH concentrations. These results suggest that PRL increase the release of corticosterone by ZFR cells via cAMP cascades and 3β‐HSD activity. J. Cell. Biochem. 73:563–572, 1999. © 1999 Wiley‐Liss, Inc.
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DOI：
10.1152/ajpcell.1998.275.1.c309
发表时间：
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期刊：
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影响因子：
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作者：
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影响因子：
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作者：
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