Protective effects of human induced pluripotent stem cell-derived exosomes on high glucose-induced injury in human endothelial cells.
Protective effects of human induced pluripotent stem cell-derived exosomes on high glucose-induced injury in human endothelial cells.
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人诱导多能干细胞来源的外泌体对高糖诱导的人内皮细胞损伤的保护作用
DOI：
10.3892/etm.2018.6059
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发表时间：
2018-06
影响因子：
2.7
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Ding Q;Sun R;Wang P;Zhang H;Xiang M;Meng D;Sun N;Chen AF;Chen S
外泌体是细胞外囊泡的一个家族，几乎所有类型的细胞都分泌外泌体，并与细胞间的通讯有关。本研究旨在探讨人诱导多能干细胞衍生外泌体（hiPSC-exo）对高糖环境下内皮细胞的细胞活力、毛细血管样结构形成和衰老的影响。从hiPSCs的条件培养基中分离外泌体，并以Alix和differentiation-63簇作为标记，通过透射电镜、纳米颗粒跟踪分析和western blot分析对外泌体进行鉴定。用PKH26标记hiPSC-exo进行跟踪，确定具有典型杯状的球形外泌体被人脐带血管内皮细胞（HUVECs）吸收。培养的HUVECs用高糖（33 mM）加或不加hiPSC-exo（20µg/ml）处理48 h，评估细胞活力、毛细血管形成和衰老情况。高糖处理显著降低HUVECs细胞活力和小管形成（P<0.0001），衰老细胞比例高于对照组（P<0.0001）。此外，hiPSC-exo恢复了高糖暴露的HUVECs的细胞活力和毛细血管样结构的形成，并减少了衰老（P<0.0001）。然而，hiPSC-exo对正常huvec的影响微乎其微。这些发现表明，干细胞来源的外泌体能够促进细胞增殖，增强毛细血管样结构的形成，并减少高糖环境下内皮细胞的衰老。
Exosomes are a family of extracellular vesicles that are secreted from almost all types of cells and are associated with cell-to-cell communication. The present study was performed to investigate the effects of human induced pluripotent stem cell-derived exosomes (hiPSC-exo) on cell viability, capillary-like structure formation and senescence in endothelial cells exposed to high glucose. Exosomes were isolated from the conditional medium of hiPSCs and confirmed by transmission electron microscopy, nanoparticle tracking analysis and western blot analysis using Alix and cluster of differentiation-63 as markers. hiPSC-exo were labeled with PKH26 for tracking, and it was determined that spherical exosomes, with a typical cup-shape, were absorbed by human umbilical vascular endothelial cells (HUVECs). Cultured HUVECs were treated with high glucose (33 mM) with or without hiPSC-exo (20 µg/ml) for 48 h, and cell viability, capillary tube formation and senescence were assessed. When exposed to high glucose, viability and tube formation in HUVECs was significantly reduced (P<0.0001), whereas the proportion of senescent cells was higher compared with that in control HUVECs (P<0.0001). Furthermore, hiPSC-exo restored cell viability and capillary-like structure formation, and reduced senescence in HUVECs exposed to high glucose (P<0.0001). However, hiPSC-exo had minimal effects on normal HUVECs. These findings suggest that stem cell-derived exosomes are able to promote cell proliferation, enhance capillary-like structure formation and reduce senescence in endothelial cells exposed to high glucose.
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影响因子：
24
作者：
Caspi, Oren;Huber, Irit;Gepstein, Lior
通讯作者：
Gepstein, Lior
影响因子：
20.3
作者：
Barres, Celine;Blanc, Lionel;Vidal, Michel
通讯作者：
Vidal, Michel
影响因子：
20.1
作者：
Jiang L;Yin M;Wei X;Liu J;Wang X;Niu C;Kang X;Xu J;Zhou Z;Sun S;Wang X;Zheng X;Duan S;Yao K;Qian R;Sun N;Chen A;Wang R;Zhang J;Chen S;Meng D
通讯作者：
Meng D
DOI：
10.1161/01.atv.11.6.1678
发表时间：
1991-11-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
BURRIG, KF
通讯作者：
BURRIG, KF
影响因子：
29.4
作者：
Mallegol, Julia;Van Niel, Guillaume;Heyman, Martine
通讯作者：
Heyman, Martine