VILLIN EXPRESSION IN THE VISCERAL ENDODERM AND IN THE GUT ANLAGE DURING EARLY MOUSE EMBRYOGENESIS
VILLIN EXPRESSION IN THE VISCERAL ENDODERM AND IN THE GUT ANLAGE DURING EARLY MOUSE EMBRYOGENESIS
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DOI：
10.1002/j.1460-2075.1988.tb03203.x
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发表时间：
1988-11-01
期刊：
影响因子：
11.4
通讯作者：
LOUVARD, D
中科院分区：
文献类型：
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作者：
MAUNOURY, R;ROBINE, S;LOUVARD, D
绒毛蛋白是一种进化上保守的Ca2+调节的肌动蛋白结合蛋白，是特化吸收细胞刷状边界的主要结构成分。利用石蜡切片和亲和纯化的多克隆抗绒毛蛋白抗体，我们研究了绒毛蛋白在小鼠胚胎发生过程中的早期表达。绒毛蛋白最早可在着床后早期在卵筒周围的内脏内胚层细胞中检测到。在这一额外的胚胎层中，绒毛蛋白的表达增加并持续到妊娠足月。在胚胎时期，绒毛首先出现在肠上，是在轴向旋转的过程中。使用相同的方法，绒毛蛋白表达也被证明在胚胎样体和畸胎瘤的区分中。在胚胎外和胚胎提取物中，绒毛蛋白的表达通过免疫印迹和Northern印迹分析得到证实，分别显示了一个93 kd的单一多肽和一个3.4 kb的mRNA长度，这是成年小鼠绒毛蛋白基因表达的两个明确参数。结果表明，石蜡切片允许在早期胚胎发生抗原非常敏感和高分辨率的检测。他们提供了绒毛蛋白表达的详细发育概况，并证明了绒毛蛋白作为参与吸收过程的上皮细胞标记物的有用性。
Villin is an evolutionarily well conserved, Ca2+ regulated actin-binding protein, and a major structural component of the brush border of specialized absorptive cells. Using paraffin sections and an affinity purified polyclonal anti-villin antibody, we have investigated the early expression of villin during mouse embryogenesis. Villin is first detectable at the early post-implantation stage in visceral endodermal cells at the periphery of the egg cylinder. In this extra embryonic layer, the expression of villin increases and then persists until full term gestation. In the embryo, villin first appears in gut anlage during the axial rotation. Using the same methodology, villin expression is also demonstrated in differentiating embryoid bodies from a teratocarcinoma. Both in extra embryonic and embryonic extracts, villin expression is confirmed by immunoblot and Northern blot analysis which reveal, respectively, a single polypeptide of 93 kd and an mRNA of 3.4 kb in length, two well defined parameters for adult mouse villin gene expression. The results presented here show that paraffin sections allow very sensitive and highly resolutive detection of antigens in early embryogenesis. They provide a detailed developmental profile of villin expression and demonstrate the usefulness of villin as a marker for epithelial cells involved in absorptive processes.