In Vitro Destruction of Streptococcus pneumoniae Biofilms with Bacterial and Phage Peptidoglycan Hydrolases
In Vitro Destruction of Streptococcus pneumoniae Biofilms with Bacterial and Phage Peptidoglycan Hydrolases
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DOI：
10.1128/aac.00492-11
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发表时间：
2011-09-01
影响因子：
4.9
通讯作者：
Moscoso, Miriam
中科院分区：
文献类型：
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作者：
Domenech, Mirian;Garcia, Ernesto;Moscoso, Miriam
宿主和噬菌体编码的细胞壁水解酶已被用于在体外以及动物模型中对抗肺炎链球菌作为嗜酸性细胞生长。然而，到目前为止，这些酶在生物膜生长的肺炎球菌中的有用性尚未得到验证。研究了肺炎链球菌产生的不同细胞壁水解酶的抗肺炎球菌活性。在体外生物膜模型中检查了肺炎克雷伯氏菌和其许多细菌。主要的肺炎球菌自溶素LytA，一种N-乙酰胞壁酰-L-丙氨酸酰胺酶，在分解S.肺炎菌生物膜。噬菌体编码的溶菌酶Cpl-1和Cpl-7也非常有效。肺炎链球菌的近亲伪链球菌和口腔链球菌形成的生物膜也被噬菌体内溶素破坏，但不被S。肺炎链球菌自溶素LytA。LytA和Cpl-1在S.记录肺炎杆菌生物膜。
Host- and phage-coded cell wall hydrolases have been used to fight Streptococcus pneumoniae growing as planktonic cells in vitro as well as in animal models. Until now, however, the usefulness of these enzymes in biofilm-grown pneumococci has gone untested. The antipneumococcal activity of different cell wall hydrolases produced by S. pneumoniae and a number of its phages was examined in an in vitro biofilm model. The major pneumococcal autolysin LytA, an N-acetylmuramoyl-L-alanine amidase, showed the greatest efficiency in disintegrating S. pneumoniae biofilms. The phage-encoded lysozymes Cpl-1 and Cpl-7 were also very efficient. Biofilms formed by the close pneumococcal relatives Streptococcus pseudopneumoniae and Streptococcus oralis were also destroyed by the phage endolysins but not by the S. pneumoniae autolysin LytA. A cooperative effect of LytA and Cpl-1 in the disintegration of S. pneumoniae biofilms was recorded.