Wounding prior to challenge substantially improves infectivity of cottontail rabbit papillomavirus and allows for standardization of infection
Wounding prior to challenge substantially improves infectivity of cottontail rabbit papillomavirus and allows for standardization of infection
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DOI：
10.1016/j.jviromet.2007.10.005
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发表时间：
2008-03-01
影响因子：
3.1
通讯作者：
Christensen, Neil D.
中科院分区：
文献类型：
--
作者：
Cladel, Nancy M.;Hu, Jiafen;Christensen, Neil D.
棉尾兔乳头瘤病毒（CRPV）/兔模型已被证明是有用的预防性和治疗性疫苗的研究和研究乳头瘤病毒感染的发病机制。它是目前唯一一种可以重现整个病毒程序的动物模型，包括癌症的进展。CRPV DNA在家兔中具有感染性，因此可以研究突变体而无需产生相应的病毒。尽管CRPV动物模型在各种实验室中广泛使用，但没有优化或标准化的方法用于产生CRPV病毒感染，特别是DNA感染。这些不同的方法使得研究人员很难比较实验室之间的结果。本文报道了一种简单高效的方法，该方法是基于以前从病毒和质粒DNA生产CRPV感染的方法进行改进的。该方法可以很容易地被该领域的其他研究人员采用。由此产生的标准化将有助于评估来自不同实验室的数据。(C)2007 Elsevier B. V.保留所有权利。
The cottontail rabbit papillomavirus (CRPV)/rabbit model has proved useful for the investigation of prophylactic and therapeutic vaccines and for the study of the pathogenesis of papillomavirus infection. It is currently the only animal model in which the entire viral program can be recapitulated, including progression to cancer. CRPV DNA is infectious in domestic rabbits and therefore mutants can be studied without the need to generate corresponding viruses. Although the CRPV animal model is used widely in various laboratories, no optimized or standardized method is used for creating CRPV` viral and especially DNA infections. These different methods have made it difficult for investigators to compare results from laboratory to laboratory. A simple and highly efficient method is reported here; it has been refined based on previous methodology for the production of CRPV infections from both virus and plasmid DNA. This method can be adapted easily by other investigators in the field. The resulting standardization will aid in the evaluation of data from different laboratories. (C) 2007 Elsevier B.V. All rights reserved.