In vitro culture conditions to study keratinocyte differentiation using the HaCaT cell line
In vitro culture conditions to study keratinocyte differentiation using the HaCaT cell line
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DOI：
10.1007/s10616-007-9076-1
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发表时间：
2007-06-01
期刊：
影响因子：
2.2
通讯作者：
Wilson, Van G.
中科院分区：
文献类型：
--
作者：
Deyrieux, Adeline F.;Wilson, Van G.
研究角质形成细胞分化过程的体外模型受到严格的培养要求和细胞固有特性的限制。原代角质形成细胞仅具有有限的寿命，而转化的细胞系表现出许多正常细胞中未发现的表型特征。自发永生化的HaCaT细胞系由于其易于繁殖和接近正常的表型而被广泛采用，但是用于分化和基因递送到HaCaT细胞中的方案在文献中变化很大。在这里，我们报告的培养条件保持HaCaT细胞在基底样状态，这些细胞的有效分化，并通过转染或腺病毒感染的转基因的交付。这份技术报告将为大量有兴趣研究分化和皮肤形态发生机制的科学家提供指导。
In vitro models to study the process of keratinocyte differentiation have been hindered by the stringent culture requirements and limitations imposed by the inherent properties of the cells. Primary keratinocytes only have a finite life span, while transformed cell lines exhibit many phenotypic features not found in normal cells. The spontaneously immortalized HaCaT cell line has been a widely employed keratinocyte model due to its ease of propagation and near normal phenotype, but protocols for differentiation and gene delivery into HaCaT cells vary widely in the literature. Here we report culture conditions for maintaining HaCaT cells in a basal-like state, for efficient differentiation of these cells, and for delivery of transgenes by transfection or adenoviral infection. This technological report will provide guidance to a large audience of scientists interested in investigating mechanisms of differentiation and skin morphogenesis.