MIDKINE IS A HEAT AND ACID STABLE POLYPEPTIDE CAPABLE OF ENHANCING PLASMINOGEN-ACTIVATOR ACTIVITY AND NEURITE OUTGROWTH EXTENSION
MIDKINE IS A HEAT AND ACID STABLE POLYPEPTIDE CAPABLE OF ENHANCING PLASMINOGEN-ACTIVATOR ACTIVITY AND NEURITE OUTGROWTH EXTENSION
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DOI：
10.1006/bbrc.1995.2661
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发表时间：
1995-11-13
影响因子：
3.1
通讯作者：
MURAMATSU, T
中科院分区：
文献类型：
--
作者：
KOJIMA, S;INUI, T;MURAMATSU, T
我们研究了一种新的肝素结合生长/分化因子中期因子（MK）的理化稳定性。发现合成的人MK是热和酸稳定的。在80 ℃下孵育90秒或在低pH下处理都不影响MK分子在高效液相色谱上的洗脱曲线。这种物理化学稳定性保持在MK的生物活性中，以增强牛内皮细胞中的纤溶酶原激活剂（PA）活性以及促进大鼠脑细胞的神经突生长。用重组鼠MK及其同源蛋白重组人多效生长因子观察到类似的稳定性。与其他几种生长因子的理化稳定性的比较表明，MK/多效生长因子是一个独特的家庭的热和酸稳定的多肽能够增强PA的活性和神经突生长。(C)1995学术出版社.
We studied about the physicochemical stability of a novel heparin-binding growth/differentiation factor, midkine (MK). It was found that synthetic human MK was heat and acid stable. Neither incubation at 80 degrees C for 90 sec nor treatment at low pH affected the elution profile of MK molecule on the high performance liquid chromatography. This physicochemical stability was maintained in the biological activities of MK to enhance plasminogen activator (PA) activity in bovine endothelial cells as well as to promote neurite outgrowth of rat brain cells. A similar stability was observed both with recombinant murine MK and with its homologous protein, recombinant human pleiotrophin. Comparison of physicochemical stability with other several growth factors suggested that MK/pleiotrophin was a unique family of heat and acid stable polypeptides capable of enhancing PA activity and neurite outgrowth. (C) 1995 Academic Press.