INHIBITORS OF RETROVIRUS INFECTION ARE SECRETED BY SEVERAL HAMSTER-CELL LINES AND ARE ALSO PRESENT IN HAMSTER SERA
INHIBITORS OF RETROVIRUS INFECTION ARE SECRETED BY SEVERAL HAMSTER-CELL LINES AND ARE ALSO PRESENT IN HAMSTER SERA
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DOI：
10.1128/jvi.67.9.5346-5352.1993
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发表时间：
1993-09-01
影响因子：
5.4
通讯作者：
MILLER, AD
中科院分区：
文献类型：
--
作者：
MILLER, DG;MILLER, AD
我们以前已经表明，中国仓鼠卵巢（CHO）细胞是抗感染的巨猿白血病病毒和嗜中性假型逆转录病毒载体，因为分泌的因子，抑制逆转录病毒感染。所测试的任何小鼠或人细胞系均不分泌此类因子。通过用糖基化抑制剂衣霉素处理CHO细胞来消除抑制剂的分泌和对感染的抗性。在这里，我们表明，对巨猿白血病病毒和嗜酸性病毒的抑制活性是部分分离的，在CHO细胞中的糖基化突变模拟衣霉素治疗的效果。我们发现，来自中国和叙利亚仓鼠的几个仓鼠细胞系分泌逆转录病毒感染的抑制剂，表明这些抑制剂不是唯一的CHO细胞系。抑制因子也存在于中国和叙利亚仓鼠的血清中，但在牛血清中未检测到。这些结果表明存在特异性因子，其功能是抑制仓鼠中的逆转录病毒感染。
We have previously shown that Chinese hamster ovary (CHO) cells are resistant to infection by gibbon ape leukemia virus and amphotropic pseudotype retroviral vectors because of the secretion of factors that inhibit retrovirus infection. Such factors were not secreted by any mouse or human cell lines tested. Secretion of the inhibitors and resistance to infection are abrogated by treatment of CHO cells with the glycosylation inhibitor tunicamycin. Here we show that the inhibitory activities against gibbon ape leukemia virus and amphotropic viruses are partially separable and that glycosylation mutations in CHO cells mimic the effects of tunicamycin treatment. We find that several hamster cell lines derived from both Chinese and Syrian hamsters secrete inhibitors of retrovirus infection, showing that these inhibitors are not unique to the CHO cell line. Inhibitory factors are also present in the sera of Chinese and Syrian hamsters but were not detected in bovine serum. These results suggest the presence of specific factors that function to inhibit retrovirus infection in hamsters.