The chaperone/usher pathways of Pseudomonas aeruginosa:: Identification of fimbrial gene clusters (cup) and their involvement in biofilm formation
The chaperone/usher pathways of Pseudomonas aeruginosa:: Identification of fimbrial gene clusters (cup) and their involvement in biofilm formation
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DOI：
10.1073/pnas.111551898
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发表时间：
2001-06-05
影响因子：
11.1
通讯作者：
Filloux, A
中科院分区：
文献类型：
--
作者：
Vallet, I;Olson, JW;Filloux, A
铜绿假单胞菌（Pseudomonasaeroginosa）是一种重要的人体机会致病菌，以生物膜的形式存在于人体的某些组织中。生物膜的形成是通过细胞间的相互作用和对表面的粘附，形成有序的结构。在这项研究中鉴定的一类突变定位在一组基因中，这些基因指定了参与其他微生物中菌毛亚基组装的伴侣/引导途径的组分。这些基因以前未在铜绿假单胞菌中描述，被命名为cupA 1-A5。在铜绿假单胞菌PAO 1的基因组中也发现了其他分子伴侣/引导系统（CupB和CupC）;然而，在表达CupA系统并在表面粘附中起作用的条件下，它们似乎在粘附中不起作用。铜绿假单胞菌细胞表面上这些假定的粘附素的鉴定表明，这种生物体具有在生物膜形成中起作用的广泛的因子。这些结构似乎是差异调节，并可能在不同阶段的生物膜形成，或在特定的环境中由这种生物体定殖功能。
Pseudomonas aeroginosa, an important opportunistic human pathogen, persists in certain tissues in the form of specialized bacterial communities, referred to as biofilm, The biofilm is formed through series of interactions between cells and adherence to surfaces, resulting in an organized structure, By screening a library of Tn5 insertions in a nonpiliated P. aeruginosa strain, we identified genes involved in early stages of biofilm formation. One class of mutations identified in this study mapped in a cluster of genes specifying the components of a chaperone/usher pathway that is involved in assembly of fimbrial subunits in other microorganisms, These genes, not previously described in P. aeruginosa, were named cupA1-A5. Additional chaperone/usher systems (CupB and CupC) have been also identified in the genome of P. aeruginosa PAO1; however, they do not appear to play a role in adhesion under the conditions where the CupA system is expressed and functions in surface adherence. The identification of these putative adhesins on the cell surface of P, aeruginosa suggests that this organism possess a wide range of factors that function in biofilm formation. These structures appear to be differentially regulated and may function at distinct stages of biofilm formation, or in specific environments colonized by this organism.