SEQUENTIAL EXPRESSION OF JUN-B, JUN-D AND FOS-B PROTEINS IN RAT SPINAL NEURONS - CASCADE OF TRANSCRIPTIONAL OPERATIONS DURING NOCICEPTION
SEQUENTIAL EXPRESSION OF JUN-B, JUN-D AND FOS-B PROTEINS IN RAT SPINAL NEURONS - CASCADE OF TRANSCRIPTIONAL OPERATIONS DURING NOCICEPTION
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DOI：
10.1016/0304-3940(91)90466-7
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发表时间：
1991-08-19
影响因子：
2.5
通讯作者：
ZIMMERMANN, M
中科院分区：
文献类型：
--
作者：
HERDEGEN, T;TOLLE, TR;ZIMMERMANN, M
用免疫细胞化学方法研究了大鼠L5脊髓中即刻早期基因编码蛋白JUN B、JUN D和FOS B的表达。 在两个实验方案中，JUN B，其不显示基础表达，在2小时后达到最大表达，此后缓慢下降。 与此相反，JUN D的表达，这是目前在许多脊髓神经元刺激前，4小时后增加，8小时后达到最大值，此后保持升高。 在基础条件下不存在的FOS B在4 h和8 h之间达到最大值，此后下降，但在24 h后仍然存在。 除JUN D外，所有免疫反应均局限于同侧背角，JUN D在8 h后也在对侧诱导。 结果进行了讨论，其意义的转录操作的JUN和FOS蛋白。
Expression of the immediate-early gene encoded proteins JUN B, JUN D and FOS B was investigated by immunocytochemistry in rat L5 spinal cord up to 24 h following stimulation of hind limb somatosensory nociceptors by noxious heat or injection of formalin. In both experimental protocols, JUN B, which did not show basal expression, reached maximum expression after 2 h and thereafter slowly decreased. In contrast, the expression of JUN D, which was present before stimulation in many spinal neurons, was increased after 4 h, reached its maximum after 8 h and thereafter remained elevated. FOS B which was absent under basal conditions reached its maximum between 4 h and 8 h and thereafter declined but was still present after 24 h. All immunoreactivities were restricted to the ipsilateral dorsal horn except JUN D which was also induced in the contralateral side after 8 h. The results are discussed in respect to their meaning for transcriptional operations of JUN and FOS proteins.