The role of UVB radiation in the induction and elicitation of photocontact hypersensitivity to TCSA in the mouse.
The role of UVB radiation in the induction and elicitation of photocontact hypersensitivity to TCSA in the mouse.
复制标题
UVB 辐射在诱导和引发小鼠对 TCSA 光接触超敏反应中的作用。
DOI：
10.1111/1523-1747.ep12533301
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
M. Kripke
中科院分区：
文献类型：
--
作者：
R. Granstein;W. Morison;M. Kripke
以环磷酰胺为免疫增强剂，可诱导小鼠对3，3 '，4'，5-四氯水杨酰苯胺（TCSA）产生光接触超敏反应（PHS）。仅UVA（320-400 nm）辐射需要敏化和诱导PHS。通过向同系小鼠注射致敏供体的淋巴结细胞，成功地将反应转移到同系小鼠中，这一发现证明了PHS的免疫学性质。UVB（280-320 nm）辐射的存在不是致敏所必需的，并且不会使PHS增加超过单独UVA辐射所观察到的水平。在所用剂量下，来自FS 40日光灯的UVB范围内的紫外线辐射（加上少量UVA辐射）没有诱导统计学上显著的PHS对TCSA，也没有在用TCSA加UVA辐射致敏的小鼠中引起显著反应。然而，在致敏前6天在远处用UVB辐射治疗小鼠抑制了PHS的诱导。这种抑制似乎类似于UVB辐射对小鼠普通接触性超敏反应的全身抑制。
Photocontact hypersensitivity (PHS) to 3,3',4',5 tetrachlorosalicylanilide (TCSA) can be induced in mice by using cyclophosphamide as an immunopotentiator. Only UVA (320-400 nm) radiation was required for both sensitization and elicitation of PHS. The reaction was successfully transferred to syngeneic mice by injecting them with lymph node cells from sensitized donors, a finding that demonstrates the immunologic nature of PHS. The presence of UVB (280-320 nm) radiation was not necessary for sensitization and did not increase PHS beyond the levels observed with UVA radiation alone. Ultraviolet radiation in the UVB range (plus a small amount of UVA radiation) from FS40 sunlamps in the dose employed did not induce statistically significant PHS to TCSA, nor did it elicit a significant response in mice sensitized with TCSA plus UVA radiation. However, treatment of mice with UVB radiation at a distant site 6 days before sensitization suppressed the induction of PHS. This suppression appeared to be analogous to the systemic suppression of ordinary contact hypersensitivity in mice by UVB radiation.