Channel catfish cytotoxic cells:: a mini-review
Channel catfish cytotoxic cells:: a mini-review
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DOI：
10.1016/s0145-305x(01)00056-8
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发表时间：
2002-03-01
影响因子：
2.9
通讯作者：
Miller, NW
中科院分区：
文献类型：
--
作者：
Shen, LL;Stuge, TB;Miller, NW
异体和自体淋巴样细胞系的使用促进了斑点叉尾轮鱼细胞毒性T淋巴细胞(CTL)和自然杀伤(NK)样细胞的研究。幼稚的鲶鱼白细胞可以自发杀伤同种异体细胞和病毒感染的自体细胞，而不需要事先致敏，体外同种异体抗原刺激可显著增强同种异体细胞毒反应。从这些同种异体抗原刺激的培养物中成功地克隆出了鲶鱼的CTL和NK样细胞，它们是第一批来自任何外温脊椎动物的细胞毒细胞系。这些克隆的细胞毒细胞含有颗粒，可能通过穿孔素/颗粒酶机制诱导敏感靶点的细胞凋亡。此外，一些鲶鱼的CTL克隆也可能通过另一种机制杀死靶标，可能是通过Fas/FasL样相互作用。重要的是，这些细胞毒细胞不表达鲶鱼非特异性细胞毒细胞(NCC)的标志，因此代表不同于NCC的细胞类型。利用抗免疫球蛋白F和G轻链亚型的单抗，揭示了某些免疫球蛋白轻链亚型的Fc受体(FcmuR)存在于某些类NK细胞上，似乎将这些细胞与表面的IgM“武装”在一起。此外，一种潜在的重要的抗鲶鱼NK样细胞的单抗(CC41)被发现能识别类似于叉尾鱼细胞毒细胞表面的150 kDa分子。这些研究清楚地表明，鲶鱼拥有一系列不同的细胞毒细胞。各种克隆的细胞毒细胞株的可获得性应该能够以目前对任何其他鱼类都不可能的方式进行明确的功能研究。(C)2001爱思唯尔科学有限公司。保留所有权利。
The use of allogeneic and autologous lymphoid cell lines has facilitated studies of cytotoxic T lymphocytes (CTL) and natural killer (NK)-like cells in channel catfish. Naive catfish leukocytes were shown to spontaneously kill allogeneic cells and virally-infected autologous cells without the need for prior sensitization, and allogeneic cytotoxic responses were greatly enhanced by in vitro alloantigen stimulation. Both catfish CTL and NK-like cells have been successfully cloned from these alloantigen-stimulated cultures, and represent the first cytotoxic cell lines derived from any ectothermic vertebrate. These cloned cytotoxic cells contain granules and likely induce apoptosis in sensititive targets via a putative perforin/granzyme mechanism. In addition, some catfish CTL clones may also kill targets by an additional mechanism, possibly by Fas/FasL-like interactions. Importantly, these cytotoxic cells do not express the marker for catfish nonspecific cytotoxic cells (NCCs), and thus represent cell types distinct from NCCs. The use of monoclonal antibodies against the catfish F and G immunoglobulin light chain isotypes revealed the presence of a putative Fc receptor for IgM (FcmuR) on some catfish NK-like cells that appears to 'arm' these cells with surface IgM. In addition, a potentially important monoclonal antibody (CC41) developed against catfish NK-like cells was found to recognize an similar to150 kDa molecule on the surface of catfish cytotoxic cells. These studies clearly demonstrate that catfish possess an array of different cytotoxic cells. The availability of various cloned cytotoxic cell lines should enable unambiguous functional studies to be performed in ways not currently possible with any other fish species. (C) 2001 Elsevier Science Ltd. All rights reserved.