Composition of the bacterial community in the gut of the pine engraver, Ips pini (Say) (Coloptera) colonizing red pine
Composition of the bacterial community in the gut of the pine engraver, Ips pini (Say) (Coloptera) colonizing red pine
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发表时间：
2007-11
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通讯作者：
Í. Delalibera;Archana Vasanthakumar;B. Burwitz;P. Schloss;K. Klepzig;J. Handelsman;K. Raffa
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作者：
Í. Delalibera;Archana Vasanthakumar;B. Burwitz;P. Schloss;K. Klepzig;J. Handelsman;K. Raffa
采用培养依赖和非培养依赖的方法，研究了松小蠹（Pine gravenger Ips pini，Say）肠道细菌群落的特征。对幼虫、蛹和成虫的单个肠道进行细菌培养，并从合并的幼虫肠道样品中提取DNA。直接从肠道菌群中扩增的16 S rRNA基因序列分析表明，与I.与许多其它系统相比，PINI相对简单。通过对幼虫、蛹和成虫肠道细菌的培养，共检出4纲6个细菌属。在I.的所有生活史中发现了两个属：泛菌属（Pantoea）和窄养单胞菌属（Stenotrophomonas）（γ-变形菌纲）。松，一致的幼虫和成虫，和不常见的蛹。在95%的采样克隆中发现了属于γ-变形菌门肠杆菌科的序列。肠杆菌科属，泛菌属和欧文氏菌属，占所有克隆序列的88%。这些结果与以前的工作一致，表明另一种树皮甲虫，南方松甲虫，Dendroctonus frontalis齐默尔曼，也有一个相对简单的肠道植物群，与木材殖民昆虫，如木材蛀虫和白蚁。对不同生活阶段的细菌组成和丰度进行了研究。松树可能与幼虫、蛹和成虫的肠道细菌群落的特定功能有关。
The gut bacterial community of a bark beetle, the pine engraver Ips pini (Say), was characterized using culture-dependent and culture-independent methods. Bacteria from individual guts of larvae, pupae and adults were cultured and DNA was extracted from samples of pooled larval guts. Analysis of 16S rRNA gene sequences amplified directly from the gut community suggests that the gut bacterial communities associated with I. pini are relatively simple, compared to many other systems. Six bacterial genera from four classes were detected by culturing gut bacteria from larvae, pupae and adults. Two genera, Pantoea and Stenotrophomonas (γ-Proteobacteria) were found in all life stages of I. pini, consistently in larvae and adults, and less commonly in pupae. Sequences that affiliate with the Enterobactereaceae of the γ-Proteobacteria were found in 95% of the clones sampled. The Enterobactereaceae genera, Pantoea and Erwinia, accounted for 88% of all clone sequences. These results are consistent with previous work indicating that another bark beetle, the southern pine beetle, Dendroctonus frontalis Zimmerman, also has a relatively simple gut flora, compared with wood colonizing insects such as wood borers and termites. The composition and abundance of bacteria associated with different life stages of I. pini are possibly associated with specific functions of the gut bacterial communities of larvae, pupae, and adults.