Low temperature and light regulate delta 12 fatty acid desaturases (FAD2) at a transcriptional level in cotton (Gossypium hirsutum).
Low temperature and light regulate delta 12 fatty acid desaturases (FAD2) at a transcriptional level in cotton (Gossypium hirsutum).
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DOI：
10.1093/jxb/ern065
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发表时间：
2008
影响因子：
6.9
通讯作者：
Farmaki T
中科院分区：
文献类型：
--
作者：
Kargiotidou A;Deli D;Galanopoulou D;Tsaftaris A;Farmaki T
脂类修饰酶在谷类等抗寒植物的抗寒性发展中起着关键作用。然而，对棉花等冷敏感物种内源酶的作用知之甚少。Δ 12脂肪酸去饱和酶（FAD 2）已知通过酰基链修饰参与对低温的适应，其用于基因表达研究以鉴定植物对低温的响应的参数。本文首次在mRNA水平上研究了低温胁迫对植物微粒体δ 12脂肪酸去饱和酶的诱导作用。定量PCR结果表明，尽管在棉花中鉴定到的Δ 12 ω 6脂肪酸去饱和酶基因FAD 2 -3和FAD 2 -4在冷胁迫下都被诱导，但FAD 2 -4的诱导显著高于FAD 2 -3。这两种亚型的诱导是光调节的，相比之下，第三种亚型FAD 2 -2不受冷或光的影响。在FAD 2 -3和FAD 2 -4基因的预测启动子中鉴定了胁迫耐受性和光调控元件。二不饱和脂肪酸种类迅速增加，从棉花叶片中分离的微粒体组分，冷胁迫。表达分析模式与所观察到的总脂肪酸和微粒体脂肪酸不饱和度水平的增加相关，表明FAD 2基因在膜适应冷应激中的直接作用。
Lipid modifying enzymes play a key role in the development of cold stress tolerance in cold-resistant plants such as cereals. However, little is known about the role of the endogenous enzymes in cold-sensitive species such as cotton. Delta 12 fatty acid desaturases (FAD2), known to participate in adaptation to low temperatures through acyl chain modifications were used in gene expression studies in order to identify parameters of plant response to low temperatures. The induction of microsomal delta 12 fatty acid desaturases at an mRNA level under cold stress in plants is shown here for first time. Quantitative PCR showed that though both delta 12 omega 6 fatty acid desaturase genes FAD2-3 and FAD2-4 identified in cotton are induced under cold stress, FAD2-4 induction is significantly higher than FAD2-3. The induction of both isoforms was light regulated, in contrast a third isoform FAD2-2 was not affected by cold or light. Stress tolerance and light regulatory elements were identified in the predicted promoters of both FAD2-3 and FAD2-4 genes. Di-unsaturated fatty acid species rapidly increased in the microsomal fraction isolated from cotton leaves, following cold stress. Expression analysis patterns were correlated with the observed increase in both total and microsomal fatty acid unsaturation levels suggesting the direct role of the FAD2 genes in membrane adaptation to cold stress.
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DOI：
10.1073/pnas.92.14.6219
发表时间：
1995-07-03
影响因子：
11.1
作者：
MOON, BY;HIGASHI, SI;MURATA, N
通讯作者：
MURATA, N
影响因子：
7.4
作者：
Nishiuchi, T;Kodama, H;Iba, K
通讯作者：
Iba, K
影响因子：
3.6
作者：
Los, DA;Ray, MK;Murata, N
通讯作者：
Murata, N
影响因子：
5.1
作者：
Berberich, T;Harada, M;Kusano, T
通讯作者：
Kusano, T
DOI：
10.1073/pnas.0502954102
发表时间：
2005-07-26
影响因子：
11.1
作者：
Ludwig, AA;Saitoh, H;Romeis, T
通讯作者：
Romeis, T