Increased osteoblast functions on undoped and yttrium-doped nanocrystalline hydroxyapatite coatings on titanium.
Increased osteoblast functions on undoped and yttrium-doped nanocrystalline hydroxyapatite coatings on titanium.
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DOI：
10.1016/j.biomaterials.2005.10.041
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发表时间：
2006-04
期刊：
影响因子：
14
通讯作者：
Michiko Sato;Marisa A. Sambito;A. Aslani;N. Kalkhoran;E. Slamovich;T. Webster
中科院分区：
文献类型：
--
作者：
Michiko Sato;Marisa A. Sambito;A. Aslani;N. Kalkhoran;E. Slamovich;T. Webster
为了提高骨科植入物的性能，本体外研究的目的是合成纳米晶羟基磷灰石（HA）粉末，以涂覆钛。采用湿化学法合成了HA。将沉淀的粉末在1100°C下烧结1小时以产生UltraCap HA（或微晶尺寸HA），或者在200°C下水热处理20小时以产生纳米晶HA。一些UltraCap和纳米晶HA粉末掺杂有钇（Y），因为先前的研究表明，与未掺杂的HA相比，本体中的Y掺杂的HA改善了成骨细胞（或骨形成细胞）的功能。采用X射线衍射（XRD）、电感耦合等离子体原子发射光谱（ICP-AES）、BET、激光粒度分析仪和扫描电子显微镜（SEM）对原始HA颗粒进行了表征。然后通过一种称为IonTite™的新型室温工艺将这些粉末沉积在钛上。将钛上所得HA涂层的性质与原始HA粉末的相应性质进行比较。结果表明，通过XRD、SEM和原子力显微镜（AFM）分析，当通过IonTite™涂覆在钛上时，原始HA粉末的化学和物理性质得以保留。更重要的是，结果显示，与传统使用的等离子喷涂HA涂层相比，纳米晶HA IonTite™涂层上的成骨细胞粘附增加。结果还表明，与UltraCap IonTite™涂层或等离子喷涂HA涂层相比，在Y掺杂纳米晶HA涂层上培养的成骨细胞的钙沉积量更大。这些结果鼓励进一步研究钛上的纳米晶IonTite™ HA涂层，以改善骨科应用。
In order to improve orthopedic implant performance, the objective of this in vitro study was to synthesize nanocrystalline hydroxyapatite (HA) powders to coat titanium. HA was synthesized through a wet chemical process. The precipitated powders were either sintered at 1100°C for 1h in order to produce UltraCap HA (or microcrystalline size HA) or were treated hydrothermally at 200°C for 20h to produce nanocrystalline HA. Some of the UltraCap and nanocrystalline HA powders were doped with yttrium (Y) since previous studies demonstrated that Y-doped HA in bulk improved osteoblast (or bone-forming cell) function over undoped HA. The original HA particles were characterized using X-ray diffraction (XRD), inductively coupled plasma–atomic emission spectroscopy (ICP–AES), BET, a laser particle size analyzer, and scanning electron microscopy (SEM). These powders were then deposited onto titanium by a novel room-temperature process, called IonTite™. The properties of the resulting HA-coatings on titanium were compared to respective properties of the original HA powders. The results showed that the chemical and physical properties of the original HA powders were retained when coated on titanium by IonTite™, as determined by XRD, SEM, and atomic force microscopy (AFM) analysis. More importantly, results showed increased osteoblast adhesion on the nanocrystalline HA IonTite™ coatings compared to traditionally used plasma-sprayed HA coatings. Results also demonstrated greater amounts of calcium deposition by osteoblasts cultured on Y-doped nanocrystalline HA coatings compared to either UltraCap IonTite™ coatings or plasma-sprayed HA coatings. These results encourage further studies on nanocrystalline IonTite™ HA coatings on titanium for improved orthopedic applications.