Haplotype Diversity of NADPH-Cytochrome P450 Reductase Gene ofOphiocordyceps sinensisand the Effect on Fungal Infection in Host Insects
Haplotype Diversity of NADPH-Cytochrome P450 Reductase Gene ofOphiocordyceps sinensisand the Effect on Fungal Infection in Host Insects
复制标题
冬虫夏草NADPH-细胞色素P450还原酶基因单倍型多样性及其对寄主昆虫真菌感染的影响
DOI：
10.3390/microorganisms8070968
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发表时间：
2020-07-01
期刊：
影响因子：
4.5
通讯作者：
Cheng, Zhou
中科院分区：
文献类型：
--
作者：
Xu, Zixian;Zhu, Yunguo;Cheng, Zhou
冬虫夏草是寄生在黑蝇科幼虫上的一种真菌寄生虫，是一种中药。然而，尚不清楚中华o.s sinensis如何感染其宿主。分析了47个中华白蚁群体219个个体的烟酰胺腺嘌呤二核苷酸磷酸(NADPH)-细胞色素P450还原酶（CPR）编码基因单倍型多样性和预测功能。在中国西部的整个分布范围内，检测到中华绒球的两个NADPH CPR基因，其优势单倍型广泛分布。只有5.43%的中华按蚊个体具有特异的NADPH单倍型CPR-1和CPR-2基因。生物信息学分析结果表明，优势基因单倍型和私有基因单倍型编码的中华按虫NADPH CPR的磷酸化位点、基序和结构域存在差异。同一个体的一对一匹配表明，具有NADPH CPR基因优势单倍型的广泛分布的中华o.s可以通过口腔或呼吸孔的随机感染，强烈感染几乎所有的寄主昆虫。相反，具有特定的NADPH CPR基因单倍型的中华按蚊，由于NADPH CPR结构和功能的改变，可能通过破坏角质层而只感染少数相应的寄主昆虫。
Ophiocordyceps sinensis Berk. is a fungal parasite that parasitizes the larvae of Hepialidae and is used as a traditional Chinese medicine. However, it is not clear how O. sinensis infects its host. The encoding gene haplotype diversity and predicted function of the nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductase (CPR) related to the fungal pathogenicity was analyzed for 219 individuals from 47 O. sinensis populations. Two NADPH CPR genes of O. sinensis were detected and their dominant haplotypes were widely distributed throughout the entire distribution range in Western China. Only 5.43% of all O. sinensis individuals possessed the specific private haplotypes of NADPH CPR-1 and CPR-2 genes. Bioinformatic analyses predicted that the phosphorylation sites, motifs, and domains of NADPH CPR of O. sinensis were different between those encoding by the dominant and private gene haplotypes. The one-to-one match fungus–host correspondence of the same individual suggested that the widely distributed O. sinensis with the dominant NADPH CPR gene haplotypes may strongly infect almost all host insects through a random infection by oral or respiratory pores. Conversely, O. sinensis with the specific private NADPH CPR gene haplotypes is likely to infect only a few corresponding host insects by breaching the cuticle, due to the changed NADPH CPR structure and function.