A detection method of CryIAc protein for identifying genetically modified rice using the lateral flow strip assay.
A detection method of CryIAc protein for identifying genetically modified rice using the lateral flow strip assay.
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一种利用侧流试纸条检测 CryIAc 蛋白鉴定转基因水稻的方法。
DOI：
10.3358/shokueishi.47.111
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
T. Maitani
中科院分区：
文献类型：
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作者：
H. Akiyama;Takahiro Watanabe;Hiroyuki Kikuchi;Kozue Sakata;S. Tokishita;Y. Hayashi;A. Hino;R. Teshima;J. Sawada;T. Maitani
我们检验了鉴定未经授权的转基因水稻的横向流动条带试验。这种转基因水稻表达苏云金芽孢杆菌（Bt）毒素、cryyac蛋白，这种蛋白具有抗虫性。从大肠杆菌包涵体中制备了重组cryac蛋白。用凝胶过滤层析法对插入cryac基因的菌株进行分析。采用横向流动条带法鉴定表达cryyiac蛋白的转基因棉花，并将其应用于添加了重组cryyiac蛋白的精米和未精米中。在糙米和精米中均能检测到0.012 μ g/g的重组cryyac蛋白。提取液上载后，需静置60分钟才能清晰检测到cryac蛋白。检测限约为每克大米12 ng CryIAc蛋白。上述结果表明，转基因棉花侧流条带法可用于检测转基因水稻中cryyac蛋白的表达。
We examined the lateral flow strip assay for identifying unauthorized genetically modified (GM) rice. The GM rice expresses the Bacillus thuringiensis (Bt) toxin, CryIAc protein, which confers tolerance to insects. The recombinant CryIAc protein was prepared from the inclusion bodies of an E. coli. strain into which the CryIAc gene had been inserted, using gel filtration chromatography. The lateral flow strip assay for the identification of GM cotton which also expresses CryIAc protein, was applied to unpolished rice and polished rice spiked with recombinant CryIAc protein. The spiked recombinant CryIAc protein was clearly detected at the level of 0.012 microg/g in both the unpolished and polished rice. After loading of the extract on the strip, a 60 -minute stand time is necessary to clearly detect CryIAc protein. The detection limit was approximately 12 ng CryIAc protein per gram of rice. These results suggest that the lateral flow strip assay for GM cotton can be used to detect CryIAc protein expressed in GM rice.
使用-seq方法研究癫痫持续状态后早期致痫阶段海马的遗传变化
DOI：
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发表时间：
2018
期刊：
影响因子：
--
作者：
小澤 千尋;河野 智海;松尾 平;小森 理絵;伊藤 康一
通讯作者：
伊藤 康一