Identification of Polyethylene Glycol-Resistant Macrophages on Stealth Imaging in Vitro Using Fluorescent Organosilica Nanoparticles
Identification of Polyethylene Glycol-Resistant Macrophages on Stealth Imaging in Vitro Using Fluorescent Organosilica Nanoparticles
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DOI：
10.1021/nn502319r
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发表时间：
2015-02-01
期刊：
影响因子：
17.1
通讯作者：
Horikawa, Kazuki
中科院分区：
文献类型：
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作者：
Nakamura, Michihiro;Hayashi, Koichiro;Horikawa, Kazuki
利用荧光有机二氧化硅纳米颗粒建立了一种体外成像系统，以评估纳米颗粒对小鼠巨噬细胞的隐身功能。采用一步法制备了具有聚乙二醇（PEG）的表面功能化有机二氧化硅纳米颗粒，得到了电刷型聚乙二醇层。同时双颗粒给药方法使我们能够利用延时荧光显微成像和流式细胞术分析来评估纳米颗粒相对于单细胞的隐身功能。单细胞成像和分析揭示了裸纳米颗粒和聚乙二醇纳米颗粒摄取的各种模式和动力学。聚乙二醇化纳米颗粒显示出对大多数巨噬细胞（聚乙二醇敏感巨噬细胞）的隐形功能；然而，对某些巨噬细胞（peg不敏感的巨噬细胞）的隐身功能未被观察到。我们鉴定并表征了具有peg抗性的巨噬细胞，它们能够以与裸纳米颗粒相同的水平吸收聚乙二醇化的纳米颗粒。
An in vitro imaging system to evaluate the stealth function of nanoparticles against mouse macrophages was established using fluorescent organosilica nanoparticles. Surface-functionalized organosilica nanoparticles with polyethylene glycol (PEG) were prepared by a one-step process, resulting in a brush-type PEG layer. A simultaneous dual-particle administration approach enabled us to evaluate the stealth function of nanoparticles with respect to single cells using time-lapse fluorescent microscopic imaging and flow cytometry analyses. Single-cell imaging and analysis revealed various patterns and kinetics of bare and PEGylated nanoparticle uptake. The PEGylated nanoparticles revealed a stealth function against most macrophages (PEG-sensitive macrophages); however, a stealth function against certain macrophages (PEG-insensitive macrophages) was not observed. We identified and characterized the PEG-resistant macrophages that could take up PEGylated nanoparticles at the same level as bare nanoparticles.