Novel substrates for tyrosinase serve as precursors of 6-hydroxydopamine and 6-hydroxydopa
Novel substrates for tyrosinase serve as precursors of 6-hydroxydopamine and 6-hydroxydopa
复制标题
酪氨酸酶的新型底物可作为 6-羟基多巴胺和 6-羟基多巴的前体
DOI：
10.1021/bi00293a001
复制
发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
G. Cohen
中科院分区：
文献类型：
--
作者：
M. Morrison;G. Cohen
Materials and MethodsMushroom tyrosinase and standards of 6-hydroxydopamine and 6-hydroxy-Dopa were obtained from Sigma. B-16 mouse melanoma tissue was used as a source of mammalian tyro-sinase; the tissue was obtained from Mason Research Institute and stored frozen over liquid nitrogen until use. For studies with mushroom tyrosinase, a solution of 1 mM substrate (2, 4-dihydroxyphenylalanine or 2, 4-dihydroxy-phenylethylamine) in 50 mM phosphate buffer, pH 6.8, con-taining 1 mM ascorbate, 50 µ L-3, 4-Dopa, and100 µ DTPA1 was incubated on a shaker bath at 22 C. The as-
