Rapid high-yield purification of canine intestinal motilin and its complete sequence determination.
Rapid high-yield purification of canine intestinal motilin and its complete sequence determination.
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犬肠胃动素的快速高产纯化及其全序列测定
DOI：
10.1016/s0021-9673(01)90460-1
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Shively,JE
中科院分区：
文献类型：
--
作者：
ReeveJr,JR;Ho,FJ;Walsh,JH;Ben-Avram,CM;Shively,JE
犬Niotilin已经从少量的犬肠道中以适合微序列分析的形式被提纯。确定的顺序为：Phe-Val-Pro-Ile-Phe-Thr-His-Ser-Glu-Leu-Gln-Lys-Ile-Arg-Glu-Lys-Glu-Arg-Asn-Lys-Ile-Arg-Asn-Lys-Gly-Gln.犬的胃动素与猪的胃动素有五个不同之处。用于犬胃动素的快速、高产量(总得率24%)显微分离技术应该适用于分离在组织中发现的低水平的其他碱性多肽，这些碱性多肽仅限量可用。这些方法应该可以更容易地实现人脑和肠肽的分离和序列测定。
Canine niotilin has been purified from small amounts of canine intestine in a form suitable for microsequence analysis. The sequence determined is: Phe-Val-Pro-Ile-Phe-Thr-His-Ser-Glu-Leu-Gln-Lys-Ile-Arg-Glu-Lys-Glu-Arg-Asn-Lys-Ile-Arg-Asn-Lys-Gly-Gln. Canine motilin differs from porcine motilin at five positions. The rapid, high-yield (24% overall yield) microisolation techniques used for canine motilin should be suitable for the isolation of other basic peptides found in low levels in tissue that is available only in limited amounts. These methods should make the isolation and sequence determination of human brain and gut peptides more readily achievable.