A-5021: A New Acyclovir Analogue Inhibits Murine Herpetic Keratitis
A-5021: A New Acyclovir Analogue Inhibits Murine Herpetic Keratitis
复制标题
A-5021：一种新的阿昔洛韦类似物可抑制小鼠疱疹性角膜炎
作者：
M. Itahashi;S. Higaki;D. Sugahara;K. Sugioka;T. Deai;Kazumasa Takao;K. Hayashi;Y. Shimomura
目的：观察一种新的阿昔洛韦类似物a -5021对小鼠疱疹性角膜炎的治疗作用。方法：在双侧BALB/c角膜上接种单纯疱疹病毒1型（CHR3株）。用裂隙灯显微镜比较A-5021滴眼液治疗组与3%阿昔洛韦眼膏治疗组角膜的临床评分。在Vero细胞单层上定量检测三叉神经节和眼球的病毒滴度。用生理盐水或白色凡士林治疗的小鼠作为对照。结果：A-5021滴眼液在所有浓度下均能显著抑制角膜上皮和间质病变。0.1% A-5021治疗的上皮和间质临床评分与3%阿昔洛韦治疗的相当。与未接受药物治疗的对照组小鼠相比，A-5021和阿昔洛韦治疗的小鼠眼球和三叉神经节中的病毒滴度明显低于对照组。结论：A-5021滴眼液易于应用于病变角膜，可改善临床评分，抑制病毒生长。这是一种有希望的替代治疗疱疹性角膜炎。
Purpose: To determine the efficacy of A-5021, a new analogue of acyclovir, on murine herpetic keratitis. Methods: Herpes simplex virus type 1 (strain CHR3) was inoculated onto bilateral scarified BALB/c corneas. Clinical scores on the corneas treated with A-5021 eyedrops were compared with those obtained from the treatment with 3% acyclovir eye ointment by slit lamp microscopy. Virus titers of the trigeminal ganglia and eyeballs were quantitated on Vero cell monolayers. Mice treated with saline or a white petroleum jelly were used as controls. Results: A-5021 eyedrops significantly suppressed both corneal epithelial and stromal lesions at all concentrations used. Clinical scores on the epithelium and stroma treated with 0.1% A-5021 were equivalent to those with 3% acyclovir treatment. When compared with the non-drug-treated control mice, virus titers in the eyeballs and trigeminal ganglia in A-5021- and acyclovir-treated mice were significantly less than those in controls. Conclusions: A-5021 eyedrops, which are easily applied onto the affected cornea, ameliorated clinical scores and suppressed virus growth. It is a promising alternative treatment of herpetic keratitis.
曲氟尿苷、西多福韦和喷昔洛韦治疗实验性疱疹性角膜炎。
DOI：
10.1001/archopht.116.6.777
发表时间：
1998
期刊：
Archives of ophthalmology (Chicago, Ill. : 1960)
影响因子：
--
作者：
Kaufman,HE;Varnell,ED;Thompson,HW
通讯作者：
Thompson,HW
影响因子：
4.4
作者：
Anand, BS;Hill, JM;Mitra, AK
通讯作者：
Mitra, AK