THE PREPARATION AND CHARACTERIZATION OF NATIVE PHOTORECEPTOR UNITS FROM THE THYLAKOIDS OF RHODOPSEUDOMONAS-VIRIDIS
THE PREPARATION AND CHARACTERIZATION OF NATIVE PHOTORECEPTOR UNITS FROM THE THYLAKOIDS OF RHODOPSEUDOMONAS-VIRIDIS
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DOI：
10.1002/j.1460-2075.1984.tb01883.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
MUHLETHALER, K
中科院分区：
文献类型：
--
作者：
JAY, F;LAMBILLOTTE, M;MUHLETHALER, K
R.绿色由规则排列的结构单元组成。每个单元由一个中心核心（被认为包含反应中心复合物）组成，周围是一个细分的蛋白质环（可能具有天线功能）。这些单独的单元可以使用各种洗涤剂处理从膜上解离。吸收光谱，作为一个标准的天然状态，被保留。所有7种主要多肽、4种反应中心多肽（细胞色素、H、M和L链）和3种捕光多肽（B1015-α，β。和γ）都在场单元的EM显示与膜内单元类似的结构。膜和单元的表面特异性碘化主要标记多肽H、B1015-α。和γ。M和L是弱标记的。B1015-.beta.在隔离单元中标记。这与其他证据一起支持将捕光多肽分配到外环。这些单元的进一步溶解将反应中心（作为含有所有4种多肽的天然复合物）与捕光多肽分离。以含有所有3种多肽和结合的色素的形式获得捕光多肽;失去了对应于天然细菌叶绿素B的1015 nm处的峰。
The photosynthetic membranes of R. viridis consist of a regular array of structural units. Each unit is composed of a central core (thought to contain the reaction center complex) surrounded by a subdivided ring of protein (of likely antennae function). These individual units can be dissociated from the membranes using a variety of detergent treatments. The absorption spectrum, used as a criterion of a native state, is retained. All of the 7 major polypeptides, the 4 reaction center polypeptides (cytochrome, H, M and L chain) and the 3 light-harvesting polypeptides (B1015-.alpha., .beta. and .gamma.) are present. EM of the units shows a similar structure to the units within the membrane. Surface-specific iodination of both membranes and units labels predominantly polypeptides H, B1015-.alpha. and .gamma.. M and L are weakly labeled. B1015-.beta. is labeled in the isolated units. This, with other evidence, supports an allocation of light-harvesting polypeptides to the outer ring. Further solubilization of these units separates the reaction center (as a native complex containing all 4 polypeptides) from the light-harvesting polypeptides. The light-harvesting polypeptides are obtained in a form containing all 3 polypeptides and bound pigment; the peak at 1015 nm corresponding to native bacteriochlorophyll b is lost.