QUALITY PROPERTIES AND EXPRESSION PROFILING OF PROTEIN DISULFIDE ISOMERASE GENES DURING GRAIN DEVELOPMENT OF THREE SPRING WHEAT NEAR ISOGENIC LINES
QUALITY PROPERTIES AND EXPRESSION PROFILING OF PROTEIN DISULFIDE ISOMERASE GENES DURING GRAIN DEVELOPMENT OF THREE SPRING WHEAT NEAR ISOGENIC LINES
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三种近等基因系春小麦籽粒发育过程中蛋白质二硫化物异构酶基因的品质特性及表达谱
DOI：
10.2298/gensr1601249d
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发表时间：
2016-01-01
影响因子：
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通讯作者：
Yan, Yueming
中科院分区：
文献类型：
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作者：
Dong, Liwei;Li, Ning;Yan, Yueming
以3个小麦谷蛋白近等基因系CB 037 A、CB 037 B和CB 037 C为材料，研究了它们的品质性状和PDI基因家族在籽粒发育过程中的转录表达谱。本研究的目的是了解不同PDI基因的动态表达与谷蛋白等位基因组成之间的关系。结果表明，麦谷蛋白等位变异对小麦主要农艺性状和产量表现无显著影响，但对小麦面筋品质有明显影响。CB 037 B的谷蛋白大聚合体（GMP）含量高于CB 037 A，面包品质优于CB 037 A，而CB 037 C的Glu-B3 h缺失显著降低了GMP含量、面团强度和面包品质。实时荧光定量聚合酶链反应（qRT-PCR）检测了8个蛋白质二硫键异构酶（PDI）基因在籽粒发育过程中的动态表达模式，结果表明，PDI 3 -1、PDI 5 -1和PDI 8 -1的高表达水平与5+ 10亚基的存在密切相关。同时，Glu-B3 h沉默导致5个PDI基因（PDI 3 -1、PDI 5 -1、PDI 6 -1、PDI 7 -2和PDI 8 -1）的表达水平显著降低，表明某些PDI基因在谷蛋白和GMP合成以及面筋品质形成中起着重要作用。
Three wheat glutenin near isogenic lines (NILs) CB037A, CB037B and CB037C were used to investigate their quality properties and the transcriptional expression profiles of PDI gene family during grain development. Our purpose is to understand the relationships between the dynamic expression of different PDI genes and glutenin allelic compositions related to gluten quality. The results showed that glutenin allelic variations had no significant effects on main agronomic traits and yield performance, but resulted in clear gluten quality changes. CB037B with 5+ 10 subunits had higher glutenin macropolymer (GMP) content and better breadmaking quality than CB037A with 2+ 12 while the lack of Glu-B3h encoding one abundant B-subunit in CB037C significantly reduced GMP content, dough strength and breadmaking quality. The dynamic expression patterns of eight protein disulfide isomerase (PDI) genes during grain development detected by quantitative real-time polymerase chain reaction (qRT-PCR) showed the close correlations between higher expression levels of PDI3-1, PDI5-1 and PDI8-1 and the presence of 5+ 10 subunits. Meanwhile, Glu-B3h silence resulted in significant decrease of expression levels of five PDI genes (PDI3-1, PDI5-1, PDI6-1, PDI7-2 and PDI8-1), suggesting the vital roles of certain PDI genes in glutenin and GMP synthesis and gluten quality formation.