NUCLEAR UPTAKE OF DNA FRAGMENTS AFTER INJECTION OF P-32-LABELLED THYMOCYTES IN LETHALLY IRRADIATED MICE
NUCLEAR UPTAKE OF DNA FRAGMENTS AFTER INJECTION OF P-32-LABELLED THYMOCYTES IN LETHALLY IRRADIATED MICE
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DOI：
10.1016/0014-4827(60)90291-3
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发表时间：
1960-01-01
影响因子：
3.7
通讯作者：
DRASIL, V
中科院分区：
文献类型：
--
作者：
HILL, M;DRASIL, V
小鼠经800 μ l全身X射线照射24小时后，经静脉注射经紫外线照射的活的或杀死的同种p32标记的胸腺细胞。对照组给予与细胞悬液活性相同的无机P32，即：e. 0.25[mu]C/小鼠。通过高分辨率放射自显影术，跟踪胸腺、脾脏、肺、肝脏、肾脏和十二指肠匀浆中放射性的器官分布。在脾和肝中，注射细胞后24和48小时的放射性保留比无机P32后更大。在胸腺、脾脏和骨髓中发现标记的胸腺细胞。注射标记细胞和无机p32后，肝脏中肝细胞的血窦、细胞质和细胞核被标记;肾脏中的肾小球毛细血管、Bowman囊和近端和远端小管;以及十二指肠中的上皮和粘膜被标记。在胸腺、脾脏和骨髓放射自显影中发现弥漫性放射性。在注射p32-胸腺细胞的小鼠样本中通过Feulgen水解除去非DNA磷后，残留放射性位于Feulgen阳性片段、淋巴细胞、网状细胞的细胞核和细胞质中，以及胸腺、脾脏和骨髓的中性粒细胞中，此外还位于股骨的骨内膜细胞中。可以得出结论，注射的胸腺细胞在淋巴器官中解体;它们的DNA的大片段被细胞质吸收，并被纳入非分裂的网状细胞和骨内膜细胞的细胞核中。
Mice, total-body X-irradiated with 800 r 24 hr previously, were injected intravenously with homologous p32-iabelled thymocytes, either living or killed by UV irradiation. The control mice were given inorganic P32 of the same activity as that of the cell suspension, i. e. 0.25 [mu]C per mouse. The organ distribution of radioactivity was followed in the homogenates of the thymus, spleen, lungs, liver, kidneys, and duodenum, and by high resolution autoradiography. There was greater retention of radioactivity at 24 and 48 hr after the injection of cells than after inorganic P32 in the spleen and liver. Labelled thymocytes were found in the thymus, spleen and bone marrow. After the injection of labelled cells as well as inorganic p32; the sinusoids, cytoplasm, and nuclei of liver cells were labelled in the liver; the glomerular capillaries, Bowman''s capsule, and both the proximal and distal tubules in the kidneys; and the epithelium and mucous membrane in the duodenum. Diffuse radioactivity was found in the thymus, spleen, and bone marrow autoradiographs. After the removal of non-DNA phosphorus by Feulgen hydrolysis in specimens of mice injected with p32- thymocytes, the residual radioactivity was localized in Feulgen positive fragments, lymphocytes, nuclei and cytoplasm of reticular cells, and in neutrophils of the thymus, spleen and bone marrow and, furthermore, in the endosteal cells of the femur. It is concluded that the injected thymocytes disintegrate in the lymphoid organs; the large fragments of their DNA are taken up by the cytoplasm and incorporated into the nuclei of the non-dividing reticular and endosteal cells.