Whole-genome comparative analysis of three phytopathogenic Xylella fastidiosa strains
Whole-genome comparative analysis of three phytopathogenic Xylella fastidiosa strains
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DOI：
10.1073/pnas.132393999
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发表时间：
2002-09-17
影响因子：
11.1
通讯作者：
Kyrpides, NC
中科院分区：
文献类型：
--
作者：
Bhattacharyya, A;Stilwagen, S;Kyrpides, NC
苛养木杆菌（Xf）在植物中引起枯萎病，并对全球主要的经济和作物损失负责。由于这种植物病原体对公众的重要性，我们开始对Xf pv柑橘的全基因组和该物种最近测序的两个菌株：Xf pv杏仁和Xf pv夹竹桃的部分基因组进行比较分析，这两个菌株分别导致杏仁和夹竹桃的叶片焦化。我们报道了对先前测序的Xf 9a5c （CVC，柑橘）菌株和两个“缺口”Xf基因组的重新分析，揭示了编码致病性和共轭转移关键功能的orf。其次，基于三个测序的Xf菌株进行详细的全基因组功能比较，确定每个菌株中存在的独特基因，以及菌株之间共有的基因。第三，对这些生物的核心功能子系统进行了“计算机”细胞重建，从而对它们的共轭传递机制进行了表征，识别了它们粘附机制的潜在差异，并强调了经典群体感应机制的缺失。这项研究证明了比较分析策略在解释密切相关的基因组中的有效性。
Xylella fastidiosa (Xf) causes wilt disease in plants and is responsible for major economic and crop losses globally. Owing to the public importance of this phytopathogen we embarked on a comparative analysis of the complete genome of Xf pv citrus and the partial genomes of two recently sequenced strains of this species: Xf pv almond and Xf pv oleander, which cause leaf scorch in almond and oleander plants, respectively. We report a reanalysis of the previously sequenced Xf 9a5c (CVC, citrus) strain and the two "gapped" Xf genomes revealing ORFs encoding critical functions in pathogenicity and conjugative transfer. Second, a detailed whole-genome functional comparison was based on the three sequenced Xf strains, identifying the unique genes present in each strain, in addition to those shared between strains. Third, an "in silico" cellular reconstruction of these organisms was made, based on a comparison of their core functional subsystems that led to a characterization of their conjugative transfer machinery, identification of potential differences in their adhesion mechanisms, and highlighting of the absence of a classical quorum-sensing mechanism. This study demonstrates the effectiveness of comparative analysis strategies in the interpretation of genomes that are closely related.