Methicillin-resistant Staphylococcus aureus (MRSA) in municipal wastewater: an uncharted threat?
Methicillin-resistant Staphylococcus aureus (MRSA) in municipal wastewater: an uncharted threat?
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DOI：
10.1111/j.1365-2672.2009.04515.x
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发表时间：
2010-04-01
影响因子：
4
通讯作者：
Lindgren, P. E.
中科院分区：
文献类型：
--
作者：
Borjesson, S.;Matussek, A.;Lindgren, P. E.
目的：(1)从污水处理厂(WWTP)中培养耐甲氧西林金黄色葡萄球菌(MRSA)；(2)研究原生MRSA菌群特征；(3)研究处理工艺对MRSA克隆分布的影响；(4)研究废水中MRSA与临床MRSA的遗传关系。对分离的MRSA进行SpA分型、药敏试验、SSCmec分型和潘通-瓦伦丁杀白素(PVL)检测。结论：所有采样均可分离到MRSA，但仅从进水口和活性污泥分离。处理工艺降低了MRSA的菌株数量和多样性，但有迹象表明，该工艺适用于耐药范围较广的菌株和PVL+菌株。本研究的意义和影响：本研究表明MRSA在废水中存活，污水处理厂可能是MRSA的潜在储存库。
Aims:(i) To cultivate methicillin-resistant Staphylococcus aureus (MRSA) from a full-scale wastewater treatment plant (WWTP), (ii) To characterize the indigenous MRSA-flora, (iii) To investigate how the treatment process affects clonal distribution and (iv) To examine the genetic relation between MRSA from wastewater and clinical MRSA.Methods:Wastewater samples were collected during 2 months at four key sites in the WWTP. MRSA isolates were characterized using spa typing, antibiograms, SSCmec typing and detection of Panton-Valentine leukocidin (PVL).Conclusions:MRSA could be isolated on all sampling occasions, but only from inlet and activated sludge. The number of isolates and diversity of MRSA were reduced by the treatment process, but there are indications that the process was selected for strains with more extensive antibiotic resistance and PVL+ strains. The wastewater MRSA-flora had a close genetic relationship to clinical isolates, most likely reflecting carriage in the community.Significance and Impact of the Study:This study shows that MRSA survives in wastewater and that the WWTP may be a potential reservoir for MRSA.