Adhesion Functions in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells
Adhesion Functions in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells
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DOI：
10.1126/science.1225399
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发表时间：
2012-10-12
期刊：
影响因子：
56.9
通讯作者：
Heisenberg, Carl-Philipp
中科院分区：
文献类型：
--
作者：
Maitre, Jean-Leon;Berthoumieux, Helene;Heisenberg, Carl-Philipp
不同的细胞粘附和皮质张力被认为是通过控制细胞-细胞接触形成来驱动细胞分选。在这里，我们表明，细胞粘附和皮质张力有不同的机械功能，在控制祖细胞-细胞接触的形成和排序在斑马鱼原肠胚。皮层张力通过调节接触处的界面张力来控制细胞-细胞接触扩张。相比之下，粘附在接触扩展中几乎没有直接作用，而是需要在其接触处机械地耦合粘附细胞的皮质，从而允许皮质张力控制接触扩展。粘附的偶联功能由E-钙粘蛋白介导，并受E-钙粘蛋白与皮质的机械锚定的限制。因此，细胞粘附为细胞皮层张力提供了机械支架，以在原肠胚形成期间驱动细胞分选。
Differential cell adhesion and cortex tension are thought to drive cell sorting by controlling cell-cell contact formation. Here, we show that cell adhesion and cortex tension have different mechanical functions in controlling progenitor cell-cell contact formation and sorting during zebrafish gastrulation. Cortex tension controls cell-cell contact expansion by modulating interfacial tension at the contact. By contrast, adhesion has little direct function in contact expansion, but instead is needed to mechanically couple the cortices of adhering cells at their contacts, allowing cortex tension to control contact expansion. The coupling function of adhesion is mediated by E-cadherin and limited by the mechanical anchoring of E-cadherin to the cortex. Thus, cell adhesion provides the mechanical scaffold for cell cortex tension to drive cell sorting during gastrulation.