Effect of the addition of a labile gelatin component on the degradation and solute release kinetics of a stable PEG hydrogel.
Effect of the addition of a labile gelatin component on the degradation and solute release kinetics of a stable PEG hydrogel.
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DOI：
10.1163/092050611x587547
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Kao WJ
中科院分区：
文献类型：
--
作者：
Waldeck H;Kao WJ
生物可降解材料的降解机制和所得产物的表征对于理解材料的行为（包括溶质传输和生物反应）至关重要。以前的数学分析的半互穿网络（sIPN）含有不稳定的明胶和稳定的交联聚（乙二醇）（PEG）网络发现，基于扩散的模型无法单独解释从系统中的溶质的释放动力学。在这项研究中，降解的sIPN及其对溶质释放和溶胀动力学的影响进行了研究。降解的主要模式，明胶溶解的动力学取决于温度，制备方法，PEGdA和明胶浓度，以及明胶和PEG之间的重量比。明胶溶出速率与基质溶胀和高分子量模型化合物FITC-葡聚糖的释放动力学均呈正相关。结合先前的体外研究，sIPN降解的动力学提供了对细胞反应（包括粘附和蛋白质表达）的时间依赖性变化的见解。这些结果提供了一个简单的指导材料配方，以控制高分子量化合物的交付与细胞行为的伴随调制。
Characterization of the degradation mechanisms and resulting products of biodegradable materials is critical in understanding the behavior of the material including solute transport and biological response. Previous mathematical analyses of a semi-interpenetrating network (sIPN) containing both labile gelatin and a stable cross-linked poly(ethylene glycol) (PEG) network found that diffusion-based models alone were unable to explain the release kinetics of solutes from the system. In this study, degradation of the sIPN and its effect on solute release and swelling kinetics were investigated. The kinetics of the primary mode of degradation, gelatin dissolution, was dependent on temperature, preparation methods, PEGdA and gelatin concentration, and the weight ratio between the gelatin and PEG. The gelatin dissolution rate positively correlated with both matrix swelling and the release kinetics of high-molecular-weight model compound, FITC-dextran. Coupled with previous in vitro studies, the kinetics of sIPN degradation provided insights into the time-dependent changes in cellular response including adhesion and protein expression. These results provide a facile guide in material formulation to control the delivery of high-molecular-weight compounds with concomitant modulation of cellular behavior.