Separation of 2-aminobenzoic acid-derivatized glycosaminoglycans and asparagine-linked glycans by capillary electrophoresis
Separation of 2-aminobenzoic acid-derivatized glycosaminoglycans and asparagine-linked glycans by capillary electrophoresis
复制标题
DOI：
10.2116/analsci.21.21
复制
发表时间：
2005-01-01
影响因子：
1.6
通讯作者：
Yamazaki, S
中科院分区：
文献类型：
--
作者：
Sato, K;Sato, K;Yamazaki, S
开发了一种毛细管电泳结合 2-氨基苯甲酸衍生化分析低聚糖的方法。使用 150 mM 硼酸盐（pH 8.5）作为运行电解质溶液，在熔融石英毛细管上有效解析糖胺聚糖 Delta-二糖。该分析方法结合酶消化应用于糖胺聚糖的鉴定。 N-聚糖或葡萄糖低聚物的分离使用含有聚乙二醇或硼酸盐作为电解质溶液的磷酸盐缓冲液进行。该方法预计可用于确定糖蛋白中的寡糖结构。
A capillary electrophoresis method was developed for the analysis of oligosaccharides combined with derivatization with 2-aminobenzoic acid. Glycosaminoglycan Delta-disaccharides were effectively resolved on a fused-silica capillary tube using 150 mM borate, pH 8.5, as a running electrolyte solution. This analytical method was applied to the identification of glycosaminoglycan in combination with enzymatic digestion. The separation of N-glycans or glucose-oligomers was performed with a phosphate buffer containing polyethylene glycol or borate as an electrolyte solution. This method is expected to be useful in the determination of oligosaccharide structures in a glycoprotein.