SELECTIVE DAMAGE OF INTRINSIC CALCITONIN GENE-RELATED PEPTIDE-LIKE IMMUNOREACTIVE ENTERIC NERVE-FIBERS IN STREPTOZOTOCIN-INDUCED DIABETIC RATS
SELECTIVE DAMAGE OF INTRINSIC CALCITONIN GENE-RELATED PEPTIDE-LIKE IMMUNOREACTIVE ENTERIC NERVE-FIBERS IN STREPTOZOTOCIN-INDUCED DIABETIC RATS
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DOI：
10.1016/0016-5085(87)90025-4
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发表时间：
1987-03-01
期刊：
影响因子：
29.4
通讯作者：
BURNSTOCK, G
中科院分区：
文献类型：
--
作者：
BELAI, A;BURNSTOCK, G
采用免疫组化技术研究了链脲霉素诱导糖尿病大鼠8周后回肠肌丛和结肠近端降钙素基因相关肽样免疫反应神经纤维的分布。糖尿病大鼠回肠和结肠近端肌肠丛神经节细胞周围的CGRP-LI神经纤维明显减少。糖尿病制剂中未见对照组中稀疏分布的免疫反应性神经细胞体。然而，CGRP-LI静脉曲张神经纤维在糖尿病肠的节间束和肌肠神经节上呈强烈染色。这些结果提示在肠壁存在双重来源的CGRP-LI神经纤维。糖尿病组织中缺乏阳性细胞体和神经节细胞周围CGRP-LI神经纤维减少，表明糖尿病状态选择性地影响内源性而非外源性的CGRP-LI神经纤维。此外，链脲霉素诱导的糖尿病大鼠肠系统P样物质免疫反应性在相同持续时间内未发生变化，提示降钙素基因相关肽和P物质存在于大鼠胃肠道内不同的神经纤维群中。
The distribution of calcitonin gene-related peptide-like immunoreactive (CGRP-LI) nerve fibers in the myenteric plexus of ileum and proximal colon of rats 8 wk after induction of diabetes with streptozotocin was studied using immunohistochemical techniques. A marked decrease in CGRP-LI nerve fibers mainly around the ganglion cells of the myenteric plexus of both ileum and proximal colon was observed in diabetic rats. The sparsely located immunoreactive nerve cell bodies in the control rats were absent in the diabetic preparations. There were, however, intensely stained CGRP-LI varicose nerve fibers that ran through the internodal strands and over the myenteric ganglia of the diabetic intestines. These findings indicate the presence of CGRP-LI nerve fibers of dual origin in the intestinal wall. The absence of positive cell bodies and diminished CGRP-LI nerve fibers around the ganglion cells in the diabetic tissues suggest that the state of diabetes selectively affects CGRP-LI nerve fibers of intrinsic rather than extrinsic origin. Furthermore, the absence of change in substance P-like immunoreactivity in the enteric system of rats with streptozotocin-induced diabetes of the same duration suggests that calcitonin gene-related peptide and substance P are contained in different populations of intrinsic nerve fibers in the gastrointestinal tract of the rat.