The complement system of elasmobranches revealed by liver transcriptome analysis of a hammerhead shark, Sphyrna zygaena
The complement system of elasmobranches revealed by liver transcriptome analysis of a hammerhead shark, Sphyrna zygaena
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DOI：
10.1016/j.dci.2016.03.009
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发表时间：
2016-08-01
影响因子：
2.9
通讯作者：
Nonaka, Masaru
中科院分区：
文献类型：
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作者：
Goshima, Masayuki;Sekiguchi, Reo;Nonaka, Masaru
对基础脊椎动物补体基因的全面研究表明，圆口类具有明显原始的补体系统，而硬骨鱼具有与哺乳动物相当的发达的补体系统。在这里，我们进行了肝脏转录组分析的锤头鲨，Sphyrna zygaeana，阐明脊椎动物补体进化的早期历史。鉴定的基因有：一个C1 q B、一个C1 r、一个C1 s、一个MASP-1/-3、一个MASP-2、两个因子B/C2、一个C3、三个C4、一个C5、一个C6、一个C7、一个C8 A、三个C8 B、一个C8 G、一个C9、两个因子I和一个S蛋白。这些结果表明，在有颌类脊椎动物的共同祖先中，存在凝集素途径、经典途径、替代途径和裂解途径。除了不存在MBL和纤维胶凝蛋白之外，MASP转录物还缺乏丝氨酸蛋白酶结构域，这表明在锤头鲨谱系中失去了凝集素途径。(C)2016爱思唯尔有限公司版权所有
Comprehensive studies of the complement genes in basal vertebrates have revealed that cyclostomes have apparently primitive complement systems whereas bony fish have well-developed complement systems comparable to those of mammals. Here we have performed liver transcriptome analysis of a hammerhead shark, Sphyrna zygaeana, to elucidate the early history of vertebrate complement evolution. Identified genes were; one C1qB, one C1r, one C1s, one MASP-1/-3, one MASP-2, two factor B/C2, one C3, three C4, one C5, one C6, one C7, one C8A, three C8B, one C8G, one C9, two factor I and one S protein. No MBL, ficolin, C1qA or C1qC were found. These results indicate that the lectin, classical, alternative and lytic pathways were established in the common ancestor of jawed vertebrates. In addition to the absence of MBL and ficolin, the MASP transcripts lacked the serine protease domain, suggesting that the lectin pathway was lost in the hammerhead shark lineage. (C) 2016 Elsevier Ltd. All rights reserved.