SPERMATID-SPECIFIC EXPRESSION OF PROTAMINE-1 IN TRANSGENIC MICE
SPERMATID-SPECIFIC EXPRESSION OF PROTAMINE-1 IN TRANSGENIC MICE
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DOI：
10.1073/pnas.84.15.5316
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发表时间：
1987-08-01
影响因子：
11.1
通讯作者：
PALMITER, RD
中科院分区：
文献类型：
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作者：
PESCHON, JJ;BEHRINGER, RR;PALMITER, RD
鱼精蛋白是一种丰富的碱性蛋白质，参与精子染色质的浓缩。在小鼠中，精蛋白基因在减数分裂后在圆形精子细胞中转录。我们已经克隆并测序了小鼠鱼精蛋白1基因。通过显微注射受精卵，产生了10系携带标记的鱼精蛋白1序列的转基因小鼠。转基因的转录仅限于圆形精子细胞，在某些情况下超过了内源基因的转录。组织特异性鱼精蛋白表达所需的顺式作用序列位于2.4-内切酶限制性片段上。利用转基因小鼠来解决雄性生殖细胞发育的基本问题的前景进行了讨论。
Protamines are abundant basic proteins involved in the condensation of sperm chromatin. In the mouse, protamine genes are transcribed postmeiotically in round spermatids. We have cloned and sequenced the mouse protamine 1 gene. Ten lines of transgenic mice harboring marked protamine 1 sequences were generated by microinjection of fertilized eggs. Transcription of the transgene is restricted to round spermatids and in several cases exceeds that of the endogenous gene. The cis-acting sequences required for tissue-specific protamine expression reside on a 2.4-kilobase restriction fragment. Prospects for using transgenic mice to address fundamental questions of male germ-cell development are discussed.