Changes in high-density lipoprotein-carried miRNA contribution to the plasmatic pool after consumption of dietary trans fat in healthy men
Changes in high-density lipoprotein-carried miRNA contribution to the plasmatic pool after consumption of dietary trans fat in healthy men
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DOI：
10.2217/epi-2016-0177
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发表时间：
2017-05-01
期刊：
影响因子：
3.8
通讯作者：
Bouchard, Luigi
中科院分区：
文献类型：
--
作者：
Desgagne, Veronique;Guerin, Renee;Bouchard, Luigi
目的：高密度脂蛋白（hdl）在循环中与心脏保护和转运功能mirna有关。本研究的目的是评估反式脂肪酸（tfa）的摄入是否会改变hdl携带的miRNA浓度及其对血浆池的贡献。方法：在一项双盲、随机交叉对照研究中，9名健康男性被喂食三种等能量饮食：第一种，富含工业tfa；第二，富含反刍动物的反式脂肪酸；第三，低tfa。从血浆中提取mirna并纯化hdl，用实时定量PCR进行定量（n = 87）。结果：7种携带hdl的mirna占血浆库的15%以上。虽然经多次检验调整后，不同饮食之间高密度脂蛋白携带的miRNA浓度没有显著差异，但不同饮食之间miR-124-3p、miR-375、miR-150-5p和miR-31-5p对血浆池的贡献发生了变化（p(FDR) < 0.05）。这些mirna在脂质代谢途径中富集。结论：这些微转录组变异可能反映了饮食对HDL功能的生理变化。
Aim: High-density lipoproteins (HDLs) are associated to cardioprotection and transport functional miRNAs in circulation. The aim of this study is to assess whether consumption of trans fatty acids (TFAs) modifies the HDL-carried miRNA concentration and their contribution to the plasmatic pool. Methods: In a double-blind, randomized crossover controlled study, nine healthy men were fed each of three isoenergetic 4-week diets: first, rich in industrial TFAs; second, rich in TFAs from ruminants; third, low in TFAs. miRNAs were extracted from plasma and purified HDLs, and quantified by the real-time quantitative PCR (n = 87). Results: Seven HDL-carried miRNAs contributed to more than 15% of the plasmatic pool. Although no significant difference in HDL-carried miRNA concentration among diets was observed after adjustment for multiple testing, changes in the contribution to the plasmatic pool between diets were observed for miR-124-3p, miR-375, miR-150-5p and miR-31-5p (p(FDR) < 0.05). These miRNAs were enriched in lipid metabolism pathways. Conclusion: These microtranscriptomic variants might reflect physiological changes in HDL functions in response to diet.