Development of miniaturized sample preparation with fibrous extraction media.
Development of miniaturized sample preparation with fibrous extraction media.
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DOI：
10.1016/j.chroma.2003.08.098
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发表时间：
2004-01
期刊：
影响因子：
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通讯作者：
Yoshihiro Saito;M. Imaizumi;K. Ban;A. Tahara;H. Wada;K. Jinno
中科院分区：
文献类型：
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作者：
Yoshihiro Saito;M. Imaizumi;K. Ban;A. Tahara;H. Wada;K. Jinno
采用聚合物细丝作为萃取介质，沿着研究了萃取毛细管的制备方法，建立了一种微型化的微柱液相色谱（micro-LC）样品制备技术。将聚合物长丝纵向填充到适当尺寸的熔融石英毛细管或聚醚醚酮（PEEK）毛细管中，并且将提取毛细管安装到微LC系统中的进样阀。在填充过程之前，应精确计数填充的细丝的数量，以确保提取毛细管制备的可重复性。与传统的开管气相色谱固定相材料，聚合物涂层的长丝的表面也进行了研究，以进一步提高萃取性能和选择性。用适合于提取特定分析物的聚合物材料涂覆，获得了提取能力的显著改善。结果表明，未来的可能性，新的定制的纤维提取介质与适当的涂层上，特别是对复杂的样品基质的分析。
Introducing fine polymeric filaments as the extraction medium, a miniaturized sample preparation technique for micro-column liquid chromatography (micro-LC) has been developed along with the investigation of a reproducible preparation scheme of the extraction capillary. The polymeric filaments were packed longitudinally into either a fused-silica capillary or a polyether ether ketone (PEEK) capillary of appropriate dimensions, and the extraction capillary was installed to the injection valve in micro-LC system. The number of packed filaments should be precisely counted before the packing process to make sure the reproducible preparation of the extraction capillary. With conventional stationary phase materials for open-tubular gas chromatography, polymeric coating to the surface of the filaments was also studied in order to further enhance the extraction performance and selectivity. Coated with the polymeric material suitable for the extraction of particular analyte, a dramatic improvement on the extraction power was obtained. The results suggest that the future possibility of novel tailored fibrous extraction medium with an appropriate coating on it, especially for the analysis of complex sample matrices.