Effects of acoustic trauma on dorsal cochlear nucleus neuron activity in slices
Effects of acoustic trauma on dorsal cochlear nucleus neuron activity in slices
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DOI：
10.1016/s0378-5955(01)00410-5
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发表时间：
2002-02-01
期刊：
影响因子：
2.8
通讯作者：
Godfrey, DA
中科院分区：
文献类型：
--
作者：
Chang, H;Chen, K;Godfrey, DA
以往的研究发现，在强噪声刺激下，动物耳蜗背核（DCN）的多单位自发活动增加，这种活动可能与耳鸣有关。我们的研究探讨了以前暴露于强烈的声音对单个神经元在DCN的影响，通过测量自发活动和敏感性乙酰胆碱，一个重要的神经递质的离心通路耳蜗核，在脑切片。自发放电记录在细胞外的DCN部分的脑切片从控制和强音暴露大鼠。暴露大鼠的切片显示爆裂的患病率增加，定期自发活动减少。由于常规神经元包括梭形细胞，而爆裂神经元包括车轮细胞，因此强音调暴露可能导致DCN车轮细胞的活性增加和梭形细胞的活性降低。或者，一些梭形的活动。细胞可能会破裂。强音暴露似乎也增加了爆裂神经元对卡巴胆碱的敏感性。这表明DCN车轮细胞自发活动的变化可能反映了强音暴露后胆碱能离心途径的影响。我们的结论是，声损伤可能会导致DCN神经元的生理和药理学的变化。这些变化可能与中枢性耳鸣的潜在机制有关。(C)2002 Elsevier Science B.V.保留所有权利。
Previous studies found increased multi-unit spontaneous activity in the dorsal cochlear nucleus (DCN) of animals that had been exposed to intense sound, Such activity may be related to tinnitus. Our study examined effects of previous exposure to intense sound on single neurons in the DCN, by measuring spontaneous activities and sensitivities to acetylcholine, an important neuro transmitter of centrifugal pathways to the cochlear nucleus, in brain slices. Spontaneous discharges were recorded extracellularly in the DCN portion of brain slices from control and intense-tone-exposed rats. Slices from exposed rats showed increased prevalence of bursting and decreased regular spontaneous activity. Since regular neurons include fusiform cells, and bursting neurons include cartwheel cells, intense tone exposure may lead to increased activity of DCN cartwheel cells and decreased activity of fusiform cells. Alternatively, the activity of some fusiform. cells might change to bursting. Intense tone exposure also appeared to increase bursting neuron sensitivity to carbachol. This suggests that changes in DCN cartwheel cell spontaneous activity may reflect changes in effects of cholinergic centrifugal pathways following intense tone exposure. We conclude that acoustic trauma may lead to changes in the physiology and pharmacology of DCN neurons. These changes may be related to underlying mechanisms of central tinnitus. (C) 2002 Elsevier Science B.V. All rights reserved.