Central and peripheral effects of physical exercise without weight reduction in obese and lean mice
Central and peripheral effects of physical exercise without weight reduction in obese and lean mice
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DOI：
10.1042/bsr20171033
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发表时间：
2018-04-27
影响因子：
4
通讯作者：
Machado de Oliveira, Camila Aparecida
中科院分区：
文献类型：
--
作者：
de Carvalho, Francine Pereira;Moretto, Thais Ludmilla;Machado de Oliveira, Camila Aparecida
旨在研究不改变体重的运动对饮食诱发肥胖 (DIO) 的中枢（下丘脑）和外周影响。十二周大的雄性 C57BI/6 小鼠接受对照 (C) 或高脂肪饮食 (H)。其中一半的人可以免费使用跑步轮，每周 5 天，持续 10 周（CE）和 HE）。评估了与能量稳态、瘦素（Stat3 和 p-Stat3）和胰岛素（Akt 和 p-Akt）信号传导相关的下丘脑基因表达。测定葡萄糖和瘦素耐量、外周胰岛素敏感性以及血浆胰岛素、瘦素和脂联素。性腺周围和腹膜后脂肪库因饮食而增加，但因运动而减少，尽管运动对体重缺乏影响。与 C 和 CE 相比，H 和 HE 中腹膜内葡萄糖耐量试验 (ipGTT) 期间的血糖较高，腹膜内胰岛素耐量试验 (ipITT) 期间的葡萄糖衰减较低。运动增加肝脏 p-Akt 表达并降低快速血糖。高脂肪饮食增加血浆胰岛素和瘦素。运动对胰岛素没有影响，但会降低瘦素并增加脂联素。瘦素抑制所有组的食物摄入。尽管 H 和 HE 小鼠血浆瘦素和胰岛素水平较高，但各组下丘脑总和 p-Stat3 和 Akt 相似。高脂肪饮食调节基因表达有利于正能量平衡。运动仅略微改变了基因表达。运动会带来积极的变化（快速血糖和脂肪储存减少；肝脏胰岛素信号传导和脂联素浓度增加），但体重不会减轻。因此，尽管减轻体重可以带来额外的好处，但当体重没有减轻时，运动的效果也不容忽视。
To investigate the central (hypothalamic) and peripheral effects of exercise without body weight change in diet-induced obesity (DIO). Twelve-week-old male C57BI/6 mice received a control (C) or a high-fat diet (H). Half of them had free access to running wheels for 5 days/week for 10 weeks (CE) and HE, respectively). Hypothalamic expression of genes related to energy homeostasis, and leptin (Stat3 and p-Stat3) and insulin (Akt and p-Akt) signaling were evaluated. Glucose and leptin tolerance, peripheral insulin sensitivity, and plasma insulin, leptin and adiponectin were determined. Perigonadal and retroperitoneal fat depots were increased by diet but reduced by exercise despite lack of effect of exercise on body weight. Blood glucose during intraperitoneal glucose tolerance test (ipGTT) was higher and glucose decay during intraperitoneal insulin tolerance test (ipITT) was lower in H and HE compared with C and CE. Exercise increased liver p-Akt expression and reduced fast glycemia. High-fat diet increased plasma insulin and leptin. Exercise had no effect on insulin but decreased leptin and increased adiponectin. Leptin inhibited food intake in all groups. Hypothalamic total and p-Stat3 and Akt were similar amongst the groups despite higher plasma levels of leptin and insulin in H and HE mice. High-fat diet modulated gene expression favoring a positive energy balance. Exercise only marginally changed the gene expression. Exercise induced positive changes (decreased fast glycemia and fat depots; increased liver insulin signaling and adiponectin concentration) without weight loss. Thus, despite reducing body weight could bring additional benefits, the effects of exercise must not be overlooked when weight reduction is not achieved.