Identification and subcellular localization of a novel mammalian dynamin-related protein homologous to yeast Vps1p and Dnm1p.
Identification and subcellular localization of a novel mammalian dynamin-related protein homologous to yeast Vps1p and Dnm1p.
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与酵母 Vps1p 和 Dnm1p 同源的新型哺乳动物动力相关蛋白的鉴定和亚细胞定位。
DOI：
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Kazuhisa Nakayama
中科院分区：
文献类型：
--
作者：
Hye;C. Shinotsuka;Seiji Torii;Kazuo Murakami;Kazuhisa Nakayama
GTP结合蛋白的动力蛋白家族参与囊泡转运。这些包括哺乳动物动力蛋白I、II、III和酵母Vps 1 p和Dnm 1 p。发动蛋白参与质膜网格蛋白包被的囊泡的形成。另一方面，Vps 1 p和Dnm 1 p似乎参与运输从后期高尔基室的液泡和内吞过程中，分别。在这项研究中，我们发现了一个新的人类蛋白质，命名为Dnm 1 p/Vps 1 p-like蛋白（DVLP）。它更接近Dnm 1 p和Vps 1 p比动力蛋白不仅在初级结构，而且在域组织。DVLP mRNA的表达无处不在，这表明这种蛋白质在细胞功能中起着重要作用。表达表位标记的DVLP的细胞的免疫荧光分析显示，它显示了扩散的核周染色模式，不叠加的标记蛋白的高尔基体，反式高尔基体网络，溶酶体，内体，或内质网。这些数据表明，DVLP不参与形成已知的包被囊泡。
The dynamin family of GTP-binding proteins are implicated in vesicular transport. These include mammalian dynamins I, II, III, and yeast Vps1p and Dnm1p. Dynamin is involved in the formation of clathrin-coated vesicles at the plasma membrane. On the other hand, Vps1p and Dnm1p appear to be involved in transport from the late Golgi compartment to vacuoles and in an endocytic process, respectively. In this study, we identified a novel human protein, named Dnm1p/Vps1p-like protein (DVLP). It resembled more closely Dnm1p and Vps1p than dynamins not only in the primary structure but also in the domain organization. DVLP mRNA was expressed ubiquitously, suggesting that this protein plays a fundamental role in cellular function. Immunofluorescence analysis of cells expressing epitope-tagged DVLP revealed that it showed a diffused perinuclear staining pattern that was not superimposed on that of the marker protein for the Golgi apparatus, trans-Golgi network, lysosomes, endosomes, or endoplasmic reticulum. These data suggest that DVLP is not involved in the formation of known coated vesicles.
DOI：
10.1073/pnas.91.2.644
发表时间：
1994-01-18
影响因子：
11.1
作者：
COOK, TA;URRUTIA, R;MCNIVEN, MA
通讯作者：
MCNIVEN, MA
高尔基体α-甘露糖苷酶II催化结构域的新纯化。
DOI：
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发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Moremen,KW;Touster,O;Robbins,PW
通讯作者：
Robbins,PW
影响因子：
11.4
作者：
WILSBACH, K;PAYNE, GS
通讯作者：
PAYNE, GS