Interaction of estrogen receptor of calf uterus with a monoclonal antibody: probing of various molecular forms.
Interaction of estrogen receptor of calf uterus with a monoclonal antibody: probing of various molecular forms.
复制标题
小牛子宫雌激素受体与单克隆抗体的相互作用：各种分子形式的探测。
DOI：
10.1021/bi00312a018
复制
发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
I. Parikh
中科院分区：
文献类型：
--
作者：
B. Moncharmont;W. L. Anderson;B. Rosenberg;I. Parikh
抗雌激素受体的单抗(JS34/32)能在小牛子宫胞浆中识别一种蛋白质(约65 000道尔顿)，在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法(SDS-PAGE)上显示一条单一条带。这种抗体的两个分子能够同时与小牛子宫胞浆中存在的天然8S形式的受体相互作用(“双胞胎抗体”试验)。这表明在受体的“低盐”8S形式上存在两个抗原决定簇。这种形式的受体显示，与可溶性抗体相互作用后，MR从345 000增加到665 000。导致8S形式解离为较小形式的解离剂也导致两个抗原决定簇的解离。雌激素受体的4S“高盐”形式每个分子有一个决定簇，似乎是受体中最小的形式，不包含与类固醇结合部位相关的重复结构。核受体还显示其分子上存在一个以上的抗原决定簇。
A monoclonal antibody to estrogen receptor (JS34/32) is able to recognize, in the calf uterine cytosol, a protein (approximately 65 000 daltons) giving a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two molecules of this antibody are able to simultaneously interact with the native 8S form of the receptor present in the calf uterine cytosol ("twin antibody" assay). This indicates the presence of two antigenic determinants on the "low-salt" 8S form of the receptor. This form of the receptor shows an increase in Mr from 345 000 to 665 000 after interaction with the soluble antibody. Dissociating agents that induce the dissociation of the 8S form to smaller forms also induce the dissociation of the two antigenic determinants. The 4S "high-salt" form of the estrogen receptor has one determinant per molecule, appearing to be the smallest form of the receptor not containing repetitive structures associated with the steroid binding site. The nuclear receptor also shows the presence of more than one antigenic determinant on its molecule.
DOI：
10.1073/pnas.77.9.5115
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GREENE, GL;NOLAN, C;JENSEN, EV
通讯作者：
JENSEN, EV