Stem-like cells in bladder cancer cell lines with differential sensitivity to cisplatin.
Stem-like cells in bladder cancer cell lines with differential sensitivity to cisplatin.
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发表时间：
2012-03
影响因子：
2
通讯作者：
M. Falso;B. Buchholz;R. D. White
中科院分区：
文献类型：
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作者：
M. Falso;B. Buchholz;R. D. White
背景膀胱癌的复发是一个常见的问题；这被归因于癌症干细胞。在这项研究中，我们鉴定了从三个对顺铂表现出不同反应的细胞系中分离出来的潜在癌症干细胞群体。材料与方法采用ALDEFLUOR®法分离TCCSUP、T24和5637细胞，观察其集落形成、分化、迁移和侵袭能力。结果细胞株表现出与顺铂耐药相关的乙醛脱氢酶高(ALDH(High))群体谱。在两个耐药细胞系T24和5637中，ALDH(High)细胞表现出更多的集落形成、迁移、侵袭和分化能力。耐药的T24和5637细胞株可作为研究膀胱癌替代疗法的模型。
BACKGROUND Recurrence is a common problem in bladder cancer; this has been attributed to cancer stem cells. In this study, we characterized potential cancer stem cell populations isolated from three cell lines that demonstrate different responses to cisplatin. MATERIALS AND METHODS The ALDEFLUOR® assay was used to isolate cells from TCCSUP, T24, and 5637 cell lines, and these cells were evaluated for their ability to form colonies, differentiate, migrate and invade. RESULTS The cell lines demonstrate a spectrum of aldehyde dehydrogenase high (ALDH(High)) populations that correlate with resistance to cisplatin. In the two resistant cell lines, T24 and 5637, the ALDH(High) cells demonstrate increased colony formation, migration, invasion, and ability to differentiate. The resistant T24 and 5637 cell lines may serve as models to investigate alternative therapies for bladder cancer.