Imaging workflow and calibration for CT-guided time-domain fluorescence tomography.
Imaging workflow and calibration for CT-guided time-domain fluorescence tomography.
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DOI：
10.1364/boe.2.003021
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发表时间：
2011-11-01
影响因子：
3.4
通讯作者：
Pogue BW
中科院分区：
文献类型：
--
作者：
Tichauer KM;Holt RW;El-Ghussein F;Zhu Q;Dehghani H;Leblond F;Pogue BW
在本研究中，概述了非接触、时间相关单光子计数小动物光学断层扫描系统的几个关键优化步骤，同时收集荧光和透射率数据。该系统用于活体时域图像重建，说明了单光子计数的灵敏度和准确处理数据所需的校准步骤。特别是，激光时间和幅度参考，检测器和滤波器校准，以及收集合适的仪器响应函数都是在时域荧光断层扫描的背景下提出的，并描述了一个全自动的工作流程。初步的幻影时域重构图像证明了基于多时间门信号的荧光断层成像工作流程的保真度。
In this study, several key optimization steps are outlined for a non-contact, time-correlated single photon counting small animal optical tomography system, using simultaneous collection of both fluorescence and transmittance data. The system is presented for time-domain image reconstruction in vivo, illustrating the sensitivity from single photon counting and the calibration steps needed to accurately process the data. In particular, laser time- and amplitude-referencing, detector and filter calibrations, and collection of a suitable instrument response function are all presented in the context of time-domain fluorescence tomography and a fully automated workflow is described. Preliminary phantom time-domain reconstructed images demonstrate the fidelity of the workflow for fluorescence tomography based on signal from multiple time gates.