Transcription of sea urchin histone genes in Escherichia coli.
Transcription of sea urchin histone genes in Escherichia coli.
复制标题
海胆组蛋白基因在大肠杆菌中的转录。
DOI：
10.1093/nar/9.16.3889
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发表时间：
1981
影响因子：
14.9
通讯作者：
K. Murray
中科院分区：
文献类型：
--
作者：
R. P. Mellado;H. Delius;B. Klein;K. Murray
通过克隆到λ置换载体中，将包含来自海胆Psammechinus miliaris和Echinus esculentus的重复组蛋白基因单元的DNA片段置于噬菌体λ启动子的控制下。虽然克隆片段中存在启动子样区域，但组蛋白基因的转录主要但不完全由λ PL启动子控制。获得了克隆的粟粒青霉组蛋白DNA片段的转录图谱。大肠杆菌组蛋白基因的顺序。esculentus是从电子显微镜分析与P.miliaris组蛋白基因的异源双链体推断的，并且与P.miliaris中的相似。在E.杆菌
DNA fragments comprising units of the repeated histone genes form the sea urchins Psammechinus miliaris and Echinus esculentus were placed under the control of bacteriophage Lambda promoters by cloning into lambda replacement vectors. Although promoter-like regions exist within the cloned fragments, transcription of the histone genes is controlled mainly, but not exclusively, by lambda PL promoter. A transcription map of the cloned P. miliaris histone DNA fragment was obtained. The order of histone genes in E. esculentus was deduced from electron microscopic analyses of heteroduplexes with P. miliaris histone genes, and is similar to that in P. miliaris. Translation products of the transcripts have not been found in E. coli.