Biogeographic distribution patterns and assembly processes of nirS-type and nirK-type denitrifiers across the black soil zone in Northeast China
Biogeographic distribution patterns and assembly processes of nirS-type and nirK-type denitrifiers across the black soil zone in Northeast China
复制标题
东北黑土区nirS型和nirK型反硝化菌生物地理分布格局及组装过程
DOI：
10.1002/saj2.20345
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发表时间：
2021
影响因子：
2.9
通讯作者：
Guanghua Wang
中科院分区：
文献类型：
--
作者：
Xiaojing Hu;Haidong Gu;Junjie Lliu;Zhuxiu Liu;Lujun Li;Shuli Du;Zhenhua Yu;Yansheng Li;Jian Jin;Xiaobing Liu;Guanghua Wang
反硝化菌控制了全球氮循环的关键步骤；然而，人们对它们的生物地理学和相关的潜在机制知之甚少。在此，基于高通量测序，我们研究了在中国东北黑土区获得的 26 个土壤样本的 thenirS 型和 nirK 型群落多样性、生物地理学、组装机制和类群相互作用。结果表明，变形菌门是已知的独特的反硝化细菌门，而缓生根瘤菌是所有土壤样本中nirS型和nirK型反硝化菌中检测到的最丰富的属。与nirS型反硝化菌相比，nirK型反硝化菌具有较高的世界性和群落多样性。这两类反硝化群落表现出明显的地理分布，并伴有明显的距离衰减关系。方差分配分析表明，土壤性质而非采样纬度对反硝化群落有显着影响，其中土壤 pH 值是主要驱动因素。然而，作为扩散限制的随机过程在决定 nirS 型和 niK 型反硝化菌的群落组装方面比确定性过程发挥了更大的作用。这种扩散限制主要是由类群之间的潜在竞争造成的，其中负相互作用在反硝化共现模式中占主导地位。此外，与其他土壤pH范围相比，土壤pH值从4.0到4.5的土壤酸化简化了网络结构，并迫使反硝化菌相互合作。这些结果为反硝化群落的生态过程提供了全面的见解，对于从微生物角度改善农业生态系统服务具有重要意义。
Denitrifiers controlled the key‐step on global N cycles; however, little knowledge had been focused on their biogeography and associated underlying mechanisms. Here, based on high‐throughput sequencing, we investigated thenirS‐type andnirK‐type community diversity, biogeography, assembly mechanisms, and taxa interactions with 26 soil samples that were obtained across the black soil zone of northeast China. The results revealed that Proteobacteria was the unique known phylum of denitrifying bacteria, andBradyrhizobiumwas the most abundant genus detected in bothnirS‐type andnirK‐type denitrifiers across all soil samples. Compared withnirS‐type denitrifiers, a relatively high cosmopolitan and community diversity were found innirK‐type. Those two types of denitrifying communities showed clear geographic distribution accompanied by obvious distance‐decay relationships. Variance partitioning analysis revealed that soil properties rather than sampling latitudes significantly influenced denitrifying communities with soil pH as the key drivers. However, stochastic processes, as dispersal limitation, played a greater role than deterministic processes in determining community assembly of bothnirS‐type andniK‐type denitrifiers. This dispersal limitation was mostly contributed by the potential competition among taxa, with the negative interactions dominating in the denitrifying co‐occurrence patterns. Moreover, soil acidification with soil pH ranking from 4.0 to 4.5 simplified network structure and compelled denitrifiers to cooperate with each other in contrast to other soil pH ranges. These results provided comprehensive insights into the ecological processes of denitrifying communities, which were of great importance to the improvement of agricultural ecosystem services from the perspective of microorganisms.