X-irradiation enhancement of transformation by benzo(a)pyrene in hamster embryo cells.
X-irradiation enhancement of transformation by benzo(a)pyrene in hamster embryo cells.
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X 射线照射增强仓鼠胚胎细胞中苯并（a）芘的转化。
DOI：
10.1073/pnas.68.8.1734
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发表时间：
1971
影响因子：
11.1
通讯作者：
R. Nelson
中科院分区：
文献类型：
--
作者：
J. DiPaolo;P. Donovan;R. Nelson
将苯并（a）芘添加到从叙利亚仓鼠胚胎培养物中获得的细胞中，这些细胞已铺板以产生离散的集落，从而导致转化。在对照中未观察到改变的菌落。仓鼠胚胎细胞的预先照射增加了每个细胞和每个总集落的苯并(a)芘转化。当照射后 48 小时添加苯并(a)芘时，出现最大增强。 250-500 R 后增强效果最大，并随着辐射剂量的增加而减弱。照射后 72 小时添加苯并(a)芘几乎没有产生增强作用。尽管转化随着苯并(a)芘浓度的增加而增加，但增强效果与所用范围内的浓度无关。在经辐射后存活的正常细胞集落中，没有发现形态发生转变的集落。
The addition of benzo(a)pyrene to cells obtained from Syrian hamster embryo cultures that had been plated to produce discrete colonies resulted in transformation. Altered colonies were not seen in controls. Prior irradiation of hamster embryo cells increased the transformation by benzo(a)pyrene, both per cell and per total colony. Maximum enhancement occurred when benzo(a)pyrene was added 48 hr after irradiation. Enhancement was greatest after 250-500 R, and decreased with higher doses of radiation. The addition of benzo(a)pyrene 72 hr after irradiation resulted in very little enhancement. Although transformation increased with benzo(a)pyrene concentration, enhancement was independent of concentration within the range used. No colony with transformed morphology was recognized among the colonies of normal cells that survived irradiation.