Temperature-dependent global gene expression in the Antarctic archaeon Methanococcoides burtonii
Temperature-dependent global gene expression in the Antarctic archaeon Methanococcoides burtonii
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DOI：
10.1111/j.1462-2920.2010.02367.x
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发表时间：
2011-08-01
影响因子：
5.1
通讯作者：
Cavicchioli, R.
中科院分区：
文献类型：
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作者：
Campanaro, S.;Williams, T. J.;Cavicchioli, R.
伯托甲烷球菌（Methanococcoides burtonii）是从南极洲艾斯湖分离出来的古细菌的成员，是研究寒冷适应的有价值的模型。全局基因表达的低温转录调控以及冷适应古菌中转录单位的排列尚未研究。我们开发了一种微阵列，用于确定哪些基因在操纵子中表达，以及哪些基因在低温（4 摄氏度）或高温（23 摄氏度）下差异表达。大约 55% 的基因被发现排列在长度从 2 到 23 个基因的操纵子中，并且 mRNA 丰度往往随着操纵子长度的增加而增加。分析其他人之前获得的盐杆菌微阵列数据揭示了操纵子长度和 mRNA 丰度之间的类似相关性，这表明操纵子可能在古细菌中更广泛地发挥类似的作用。超过 500 个基因的差异表达水平高达 24 倍。一个显着的特征是参与维持RNA处于适合寒冷翻译状态的基因的上调。微阵列实验与先前使用蛋白质组学获得的结果之间的比较表明，转录调节（而不是翻译）主要负责控制伯氏巨藻中的基因表达。此外，某些基因（例如参与核糖体结构和产甲烷作用）似乎受到转录后调节。这是描述古细菌操纵子全基因组分布和调控的少数实验研究之一。
Methanococcoides burtonii is a member of the Archaea that was isolated from Ace Lake in Antarctica and is a valuable model for studying cold adaptation. Low temperature transcriptional regulation of global gene expression, and the arrangement of transcriptional units in cold-adapted archaea has not been studied. We developed a microarray for determing which genes are expressed in operons, and which are differentially expressed at low (4 degrees C) or high (23 degrees C) temperature. Approximately 55% of genes were found to be arranged in operons that range in length from 2 to 23 genes, and mRNA abundance tended to increase with operon length. Analysing microarray data previously obtained by others for Halobacterium salinarum revealed a similar correlation between operon length and mRNA abundance, suggesting that operons may play a similar role more broadly in the Archaea. More than 500 genes were differentially expressed at levels up to similar to 24-fold. A notable feature was the upregulation of genes involved in maintaining RNA in a state suitable for translation in the cold. Comparison between microarray experiments and results previously obtained using proteomics indicates that transcriptional regulation (rather than translation) is primarily responsible for controlling gene expression in M. burtonii. In addition, certain genes (e. g. involved in ribosome structure and methanogenesis) appear to be regulated post-transcriptionally. This is one of few experimental studies describing the genome-wide distribution and regulation of operons in archaea.